Split-luciferase complementation assay to detect channel-protein interactions in live cells.
Split-luciferase complementation assay to detect channel-protein interactions in live cells.
复制标题
裂解荧光素酶互补测定可检测活细胞中的通道蛋白相互作用。
DOI：
10.1007/978-1-4939-2425-7_33
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Laezza,Fernanda
中科院分区：
文献类型：
--
作者：
Shavkunov,AlexanderS;Ali,SyedR;Panova-Elektronova,NeliI;Laezza,Fernanda
The understanding of ion channel function continues to be a significant driver in molecular pharmacology. In this field of study, protein-protein interactions are emerging as fundamental molecular determinants of ion channel function and as such are becoming an attractive source of highly specific targets for drug development. The investigation of ion channel macromolecular complexes, however, still relies on conventional methods that are usually technically challenging and time-consuming, significantly hampering our ability to identify, characterize and modify ion channel function through targeted molecular approaches. As a response to the urgent need of developing rapid and albeit accurate technologies to survey ion channel molecular complexes, we describe a new application of the split-luciferase complementation assay to study the interaction of the voltage-gated Na + channel with the intracellular fibroblast growth factor 14 and its dynamic regulation in live cells. We envision that the flexibility and accessibility of this assay will have a broad impact in the ion channel field complementing structural and functional studies, enabling the interrogation of protein–channel dynamic interactions in complex cellular contexts and laying the basis for new frameworks in drug discovery campaigns.
