LC-ESI-MS Determination of Imperatorin in Rat Plasma After Oral Administration and Total Furocoumarins of Radix Angelica dahuricae and its Application to a Pharmacokinetic Study
LC-ESI-MS Determination of Imperatorin in Rat Plasma After Oral Administration and Total Furocoumarins of Radix Angelica dahuricae and its Application to a Pharmacokinetic Study
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LC-ESI-MS测定白芷口服后大鼠血浆中欧前胡素和总呋喃香豆素含量及其在药动学研究中的应用
DOI：
10.1365/s10337-009-1024-0
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发表时间：
2009-05-01
期刊：
影响因子：
1.7
通讯作者：
Li, Hao
中科院分区：
文献类型：
--
作者：
Zhang, Jiye;Yang, Guangde;Li, Hao
建立了一种简单、快速、灵敏、可靠的液相色谱-电喷雾电离质谱法测定大鼠口服白芷血浆中欧前胡素和总呋喃香豆素含量的方法。血浆样品加入乙腈制得的内标蛇床子碱溶液去蛋白。分析采用Shim-pack C-18色谱柱（150 × 2.0 mm, 5 μ m），乙腈和0.5%甲酸溶液（70:30,v/v）为流动相。在四极质谱检测器上进行检测，该检测器具有ESI接口，在选定的离子监测模式下工作。方法在大鼠血浆中线性定量范围为2 ~ 4000 ng mL(-1)，相关系数大于0.99，检出限（LOD）为0.5 ng mL(-1)，定量下限（LLOQ）为2 ng mL（-1）。日内、日间相对标准偏差（RSD）分别小于2.5和3.5%。回收率均在90%以上。该方法成功地应用于口服白芷总呋喃香豆素和欧前胡素在大鼠体内的药动学研究。
A simple, rapid, sensitive and reliable liquid chromatography-electrospray ionization mass spectrometry method for the quantification of imperatorin in rat plasma after oral administration and total furocoumarins of Radix Angelica dahuricae has been established. The plasma samples were deproteinized by adding internal standard (IS) osthole solution, which was prepared by acetonitrile. The analysis was performed on a Shim-pack C-18 column (150 x 2.0 mm i.d., 5 mu m) using acetonitrile and 0.5% formic acid solution (70:30, v/v) as a mobile phase. The detection was performed on a quadrupole mass spectrometer detector with an ESI interface operated in the selected ion monitoring mode. The linear quantification range of the method was 2-4000 ng mL(-1) in rat plasma with a correlation coefficient greater than 0.99, the limit of detection (LOD) was 0.5 ng mL(-1) and the lower limit of quantification (LLOQ) 2 ng mL(-1). The intra- and inter-day relative standard deviations (RSD) were less than 2.5 and 3.5%, respectively. The recoveries were above 90%. The validated method was successfully applied to a pharmacokinetic study of imperatorin in rats after oral administration and total furocoumarins of Radix Angelica dahuricae.