IS HEMOGLOBIN AN ESSENTIAL STRUCTURAL COMPONENT OF HUMAN ERYTHROCYTE MEMBRANES
IS HEMOGLOBIN AN ESSENTIAL STRUCTURAL COMPONENT OF HUMAN ERYTHROCYTE MEMBRANES
复制标题
DOI：
10.1172/jci104747
复制
发表时间：
1963-01-01
影响因子：
15.9
通讯作者：
REED, CF
中科院分区：
文献类型：
--
作者：
WEED, RI;BERG, G;REED, CF
用Danon的逐渐渗透裂解技术制备了99.95%不含血红蛋白的人红细胞膜，发现它含有完整细胞的所有脂质。此外，膜atp酶保持完整，幽灵保持其双凹形状和渗透反应性。这些结果被解释为血红蛋白在膜中没有结构作用。
Human erythrocyte membranes (ghosts) which are 99.95% free of hemoglobin have been prepared by Danon''s technique of gradual osmotic lysis and found to contain all of the lipids of the intact cell. In addition, membrane ATPase remains intact and the ghosts retain their biconcave shape and osmotic responsiveness. These results are interpreted as indicating no structural role for hemoglobin in the membrane.