3D printed tricalcium phosphate scaffolds: Effect of SrO and MgO doping on in vivo osteogenesis in a rat distal femoral defect model.
3D printed tricalcium phosphate scaffolds: Effect of SrO and MgO doping on in vivo osteogenesis in a rat distal femoral defect model.
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3D印刷三卡磷磷酸三氯磷酸酯支架：SRO和MGO兴奋剂对大鼠股骨远端缺陷模型中体内成骨的影响。
DOI：
10.1039/c3bm60132c
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发表时间：
2013-12-01
影响因子：
6.6
通讯作者：
Bose S
中科院分区：
文献类型：
--
作者：
Tarafder S;Davies NM;Bandyopadhyay A;Bose S
互连大孔的存在在用于引导组织再生的组织工程支架中是重要的。本研究报告了由于在磷酸三钙（TCP）中添加SrO和MgO作为掺杂剂，互连的大孔磷酸三钙（TCP）支架的体内生物性能。我们使用直接三维打印（3DP）技术进行支架制造，然后进行微波烧结。通过具有500 µm、750 µm和1000 µm互连设计孔径的支架评估机械强度。对于500 µm互连设计孔径Sr-Mg掺杂支架，实现了12.01 ± 1.56 MPa的最大压缩强度。通过在大鼠股骨远端缺损中植入350 μm设计的互连大孔支架来评估微波烧结的纯TCP和Sr-Mg掺杂的TCP支架的体内生物学性能。对于纯的和SrO-MgO掺杂的TCP支架，这些3D打印支架的烧结孔径分别为311 ± 5.9 μm和245 ± 7.5 μm。这些3D打印支架具有多尺度孔隙率，即，三维互连设计的大孔沿着与固有的微孔。组织形态学和组织形态计量学分析显示，与纯TCP支架相比，类骨质样新骨形成显著增加，并且SrO和MgO掺杂的3D打印TCP支架内的矿化加速。与纯TCP支架相比，SrO和MgO掺杂的TCP支架在大鼠血清中也观察到骨钙素和I型胶原水平的增加。我们的研究结果表明，这些3D打印的SrO和MgO掺杂的TCP支架具有多尺度孔隙率，通过加速骨生成有助于早期愈合。
The presence of interconnected macro pores is important in tissue engineering scaffolds for guided tissue regeneration. This study reports in vivo biological performance of interconnected macro porous tricalcium phosphate (TCP) scaffolds due to the addition of SrO and MgO as dopants in TCP. We have used direct three dimensional printing (3DP) technology for scaffold fabrication followed by microwave sintering. Mechanical strength was evaluated by scaffolds with 500 µm, 750 µm, and 1000 µm interconnected designed pore sizes. Maximum compressive strength of 12.01 ± 1.56 MPa was achieved for 500 µm interconnected designed pore size Sr-Mg doped scaffold. In vivo biological performance of the microwave sintered pure TCP and Sr-Mg doped TCP scaffolds was assessed by implanting 350 µm designed interconnected macro porous scaffolds in rat distal femoral defect. Sintered pore size of these 3D printed scaffolds were 311 ± 5.9 µm and 245 ± 7.5 µm for pure and SrO-MgO doped TCP scaffolds, respectively. These 3D printed scaffolds possessed multiscale porosity, i.e., 3D interconnected designed macro pores along with intrinsic micro pores. Histomorphology and histomorphometric analysis revealed a significant increase in osteoid like new bone formation, and accelerated mineralization inside SrO and MgO doped 3D printed TCP scaffolds as compared to pure TCP scaffolds. An increase in osteocalcin and type I collagen level was also observed in rat blood serum with SrO and MgO doped TCP scaffolds compared to pure TCP scaffolds. Our results show that these 3D printed SrO and MgO doped TCP scaffolds with multiscale porosity contributed to early healing through accelerated osteogenesis.