Actively transcribed genes in the raf oncogene group, located on the X chromosome in mouse and human.
Actively transcribed genes in the raf oncogene group, located on the X chromosome in mouse and human.
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DOI：
10.1073/pnas.83.11.3934
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发表时间：
1986-06
影响因子：
11.1
通讯作者：
K. Huebner;A. Ar-rushdi;C. Griffin;M. Isobe;C. Kozak;B. Emanuel;L. Nagarajan;J. Cleveland;T. Bonner;M. Goldsborough
中科院分区：
文献类型：
--
作者：
K. Huebner;A. Ar-rushdi;C. Griffin;M. Isobe;C. Kozak;B. Emanuel;L. Nagarajan;J. Cleveland;T. Bonner;M. Goldsborough
与c-raf-1相关但不同的鼠和人cDNA已被分离并分别命名为mA-raf和hA-raf。mA-raf和hA-raf cDNA在限制酶切割的鼠和人细胞DNA的Southern印迹中检测到相同的鼠和人片段。与mA-raf cDNA同源的小鼠限制性内切酶片段在一组中国仓鼠-小鼠杂交细胞中与小鼠X染色体共分离，从而将mA-raf位点定位于小鼠X染色体。由hA-raf cDNA（或mA-raf cDNA）检测到的两个独立分离的基因座hA-raf-1和hA-raf-2分别位于人类X和7号染色体上。mA-raf基因座和hA-raf-1基因座在几种小鼠和人细胞系中活跃转录。
Murine and human cDNAs, related to but distinct from c-raf-1, have been isolated and designated mA-raf and hA-raf, respectively. The mA-raf and hA-raf cDNAs detect the same murine and human fragments in Southern blots of restriction enzyme-cleaved murine and human cellular DNA. The murine restriction enzyme fragments homologous to mA-raf cDNA cosegregate with mouse chromosome X in a panel of Chinese hamster-mouse hybrid cells, thus localizing the mA-raf locus to mouse chromosome X. Two independently segregating loci, detected by the hA-raf cDNA (or mA-raf cDNA), hA-raf-1 and hA-raf-2, are located on human chromosomes X and 7, respectively. The mA-raf locus and the hA-raf-1 locus are actively transcribed in several mouse and human cell lines.