DARPP-32, A PHOSPHOPROTEIN ENRICHED IN DOPAMINOCEPTIVE NEURONS BEARING DOPAMINE D1 RECEPTORS - DISTRIBUTION IN THE CEREBRAL-CORTEX OF THE NEWBORN AND ADULT RHESUS-MONKEY
DARPP-32, A PHOSPHOPROTEIN ENRICHED IN DOPAMINOCEPTIVE NEURONS BEARING DOPAMINE D1 RECEPTORS - DISTRIBUTION IN THE CEREBRAL-CORTEX OF THE NEWBORN AND ADULT RHESUS-MONKEY
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DOI：
10.1002/cne.902990306
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发表时间：
1990-09-15
影响因子：
2.5
通讯作者：
GOLDMANRAKIC, PS
中科院分区：
文献类型：
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作者：
BERGER, B;FEBVRET, A;GOLDMANRAKIC, PS
DARPP - 32是一种多巴胺（DA）和环腺苷酸调节的磷蛋白，与基底神经节中带有D - 1受体的多巴胺感受神经元相关。本研究探讨了DARPP - 32在灵长类大脑皮层中的分布及其与该结构中富含D - 1受体细胞的可能关联。对3日龄（P3）、6周龄（P42）和成年恒河猴的大脑皮层中的DARPP - 32样免疫反应（LIR）神经元进行了检测。在较年幼的个体中，在整个皮层的V - VI层观察到大量具有锥体细胞形态特征的DARPP - 32阳性神经元，在II层和III层最上部也有少量分布。在顶叶、岛叶、颞叶和枕叶皮层中，DARPP - 32阳性神经元在Va层呈单层排列。它们经常成小群聚集，其树突成束。在初级运动皮层中，贝茨细胞在标记的细胞群中。在联合区和躯体感觉区，DARPP - 32阳性神经元的基底树突以及其在I层的显著的顶树突簇形成一种基本的双层模式，类似于这些区域中多巴胺传入纤维和D - 1受体所报道的分布。V层中DARPP - 32阳性神经元的显著和广泛分布可能是灵长类皮层的一种特化，因为在啮齿动物中此类细胞仅在有限的位置被发现。关于大脑皮层连接的文献表明，VI层甚至Va层中的大量DARPP - 32阳性神经元可能是皮质丘脑神经元。一个重要的发育观察结果是在白质中存在DARPP - 32 - LIR神经元。它们在新生儿中显著，但在成年个体中无法看到。它们的位置以及类型和形状使人联想到间质神经元。在成年猴子中，DARPP - 32 - LIR神经元的分布更局限：它们在颞叶腹侧回以及与边缘系统相关的区域数量众多：眶额皮质尾部、岛叶、颞极、内嗅皮层和前扣带皮层。在上颞叶和顶叶皮层的Va层、一些前额叶区域（10、13和内侧9）以及运动前区和辅助运动皮层中检测到弱标记；与新生儿不同，在成年个体中，背外侧前额叶皮层、初级运动皮层或初级视觉皮层或纹状前皮层中几乎没有DARPP - 32 - LIR神经元。这些发现表明在出生和成年之间DARPP - 32的表达存在重要的发育变化，并指出该分子和/或D - 1受体在灵长类大脑皮层出生后发育过程中可能的作用。
DARPP-32, a dopamine (DA) and cAMP-regulated phosphoprotein, is associated with dopaminoceptive neurons bearing D-1 receptors in the basal ganglia. The present study addressed the distribution of DARPP-32 in the primate cerebral cortex and its putative association with D-1 receptor laden cells in this structure. DARPP-32-like immunoreactive (LIR) neurons were examined in the cerebral cortex of 3-day-old (P3), 6-week-old (P42), and adult rhesus monkeys. In the younger cases, a large number of DARPP-32 positive neurons, with the morphological characteristics of pyramidal cells, were observed throughout the cortex, in layers V-VI, and to a lesser extent in layer II and uppermost layer III. In the parietal, insular, temporal, and occipital cortices, DARPP-32 positive neurons were arranged in a monolayer in layer Va. They were often clustered in small groups with a bundling of their dendrites. In the primary motor cortex, Betz cells were among the labeled population. In the association and somatosensory areas, the basal dendrites of DARPP-32 positive neurons and the prominent tufting of their apical dendrites in layer I contributed to an essential bilaminar pattern resembling the distribution reported for DA afferents and D-1 receptors in these areas. The prominence and widespread distribution of DARPP-32 positive neurons in layer V may be a specialization of primate cortex since such cells are found only in restricted locations in rodents. The literature on the connections of the cerebral cortex suggests that a large number of the DARPP-32 positive neurons in layer VI and perhaps even in layer Va may be corticothalamic neurons. An important developmental observation was the presence of DARPP-32-LIR neurons in the white matter. They were prominent in the neonates but could not be seen in the adult. Their location as well as their type and shape were reminiscent of interstitial neurons. In the adult monkeys, the distribution of DARPP-32-LIR neurons was more circumscribed: they were numerous in the ventral temporal gyrus and in areas related to the limbic system: caudal orbitofrontal cortex, insula, temporal pole, entorhinal, and anterior cingulate cortex. Weak labeling was detected in layer Va of the superior temporal and parietal cortex, in some prefrontal areas (10, 13, and medial 9), and in the premotor and supplementary motor cortex; in adults, unlike neonates, few DARPP-32-LIR neurons were present in the dorsolateral prefrontal cortex, the primary motor or the primary visual or prestriate cortices. These findings demonstrate important developmental changes between birth and adulthood in the expression of DARPP-32 and point to a possible role for this molecule and/or D-1 receptors during postnatal development of the primate cerebral cortex.