Bacterial vaginosis: culture- and PCR-based characterizations of a complex polymicrobial disease's pathobiology.
Bacterial vaginosis: culture- and PCR-based characterizations of a complex polymicrobial disease's pathobiology.
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DOI：
10.1007/s11908-007-0074-4
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发表时间：
2007-11-01
影响因子：
3.1
通讯作者：
Akins, R A
中科院分区：
文献类型：
--
作者：
Kalra, Apoorv;Palcu, Cristina T;Akins, R A
细菌性阴道病（BV）是一种神秘的多微生物疾病，其进化和病理生物学无法通过传统的基于培养的方法来解决。通过基于聚合酶链反应的方法来表征阴道微生物群具有很大的前景。分子研究已经鉴定出了培养不能检测到的物种，但也遗漏了一些培养不能识别的物种。这些研究允许根据不同的微生物特征对正常和细菌性阴道炎患者进行分类，这可能对了解细菌性阴道炎的风险、对治疗的反应和并发症的风险很重要。需要更多使用新一代引物和标准化方法的研究，并且必须根据微生物谱对患者进行分组后对数据进行分析。
Bacterial vaginosis (BV) is an enigmatic polymicrobial disease, and its evolution and pathobiology will not be solved by traditional culture-based methods. Characterization of the vaginal microbiota by polymerase chain reaction-based methods holds great promise. Molecular studies have identified species not detected by culture, but they also have missed some species identified by culture. These studies allow classification of both normal and BV patients based on distinct microbiologic profiles, which may prove important in accessing risk of BV, response to treatment, and risk of complications. More studies using new generations of primers and standardized methods are needed, and data must be analyzed after grouping patients according to microbiologic profiles.