Fluorescent protein tracking and detection: applications using fluorescent proteins in living cells.
Fluorescent protein tracking and detection: applications using fluorescent proteins in living cells.
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DOI：
10.1101/pdb.top64
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发表时间：
2009-12-01
影响因子：
--
通讯作者：
Piston, David W
中科院分区：
文献类型：
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作者：
Rizzo, Mark A;Davidson, Michael W;Piston, David W
荧光蛋白（FP）是一种用途广泛的成像探针，已成功应用于从微生物学到系统生理学的几乎所有生物学科。它们作为培养细胞、离体组织和整个动物中基因表达研究的报告基因非常有用。在这篇文章中，FP应用的讨论将集中在活细胞显微镜技术。在活细胞中，FP最常用于跟踪蛋白质、细胞器和其他细胞区室的定位和动态。FP也可用于通过使用荧光共振能量转移（FRET）来评估活细胞中的蛋白质-蛋白质相互作用。这篇文章提供了一些一般性的提示，使用和成像增强型绿色荧光蛋白（EGFP）和调色板的新成员，以及定量成像的FP和成像的几个FP在同一时间的实际方面。最后，概述了不同类型的生物传感器，已来自FP。
Fluorescent proteins (FPs) are quite versatile imaging probes and have been successfully employed in almost every biological discipline from microbiology to systems physiology. They are extremely useful as reporters for gene-expression studies in cultured cells, excised tissues, and whole animals. In this article, the discussion of FP applications will be focused on live cell microscopy techniques. In live cells, FPs are most commonly used to track the localization and dynamics of proteins, organelles, and other cellular compartments. FPs can also be used to assess protein-protein interactions in living cells through the use of fluorescence resonance energy transfer (FRET). This article provides some general tips for the practical aspects of using and imaging enhanced green fluorescent protein (EGFP) and newer members of the color palette, as well as quantitative imaging of FPs and imaging of several FPs at the same time. Finally, an overview is provided for the different types of biosensors that have been derived from FPs.