A multifunctional polyketide-peptide synthetase essential for albicidin biosynthesis in Xanthomonas albilineans.
A multifunctional polyketide-peptide synthetase essential for albicidin biosynthesis in Xanthomonas albilineans.
复制标题
一种多功能聚酮肽合成酶，对于白色黄单胞菌中白杀菌素生物合成至关重要。
作者：
Guozhong Huang;Lian;Lian;R. G. Birch
白菌素（Albicidins）是甘蔗叶片烫伤病原菌白色黄单胞菌（Xanthomonas albilineans）产生的一类强效抗生素和植物毒素，可抑制细菌和质体的DNA复制。一个由tn5标记定位的基因通过互补被确认参与白孢杆菌素的生物合成。该基因（xabB）编码一个大蛋白（预测M:(r) 525695），具有模块化结构，表明多功能聚酮合成酶（PKS）与非核糖体肽合成酶（NRPS）相连。XabB全长4801个氨基酸，是目前报道的最大的PKS-NRPS。该多功能酶的12个催化结构域排列顺序为：N端-酰基辅酶a连接酶(AL)-酰基载体蛋白(ACP)- β -酮酰基合成酶(KS)- β -酮酰基还原酶(KR)-ACP-ACP-KS-肽基载体蛋白(PCP)-缩合(C)-腺苷化-PCP-C。XabB的模块化结构表明了白菌素生物合成的可能步骤和提高抗生素产量的方法。与已知的用于抗生素生产的PKS-NRPS酶相比，这种新的结构域模式也有助于合理设计生产新型抗生素的酶的知识库。
Albicidins, a family of potent antibiotics and phytotoxins produced by the sugarcane leaf scald pathogen Xanthomonas albilineans, inhibit DNA replication in bacteria and plastids. A gene located by Tn5-tagging was confirmed by complementation to participate in albicidin biosynthesis. The gene (xabB) encodes a large protein (predicted M:(r) 525695), with a modular architecture indicative of a multifunctional polyketide synthase (PKS) linked to a non-ribosomal peptide synthetase (NRPS). At 4801 amino acids in length, XabB is the largest reported PKS-NRPS. Twelve catalytic domains in this multifunctional enzyme are arranged in the order N terminus-acyl-CoA ligase (AL)-acyl carrier protein (ACP)-beta-ketoacyl synthase (KS)-beta-ketoacyl reductase (KR)-ACP-ACP-KS-peptidyl carrier protein (PCP)-condensation (C)-adenylation-PCP-C. The modular architecture of XabB indicates likely steps in albicidin biosynthesis and approaches to enhance antibiotic yield. The novel pattern of domains, in comparison with known PKS-NRPS enzymes for antibiotic production, also contributes to the knowledge base for rational design of enzymes producing novel antibiotics.
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