Dextran-stabilized Fe-Mn bimetallic oxidase-like nanozyme for total antioxidant capacity assay of fruit and vegetable food
Dextran-stabilized Fe-Mn bimetallic oxidase-like nanozyme for total antioxidant capacity assay of fruit and vegetable food
复制标题
葡聚糖稳定的铁锰双金属氧化酶类纳米酶用于果蔬食品总抗氧化能力测定
DOI：
10.1016/j.foodchem.2021.131115
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发表时间：
2021-09-20
期刊：
影响因子：
8.8
通讯作者：
Zhang, Wentao
中科院分区：
文献类型：
--
作者：
Han, Ximei;Liu, Ling;Zhang, Wentao
总抗氧化能力（TAC）对于评估医疗保健领域的抗氧化食品质量变得越来越重要。本文根据食品中存在的各种抗氧化剂与 Dex-FeMnzyme/oxTMB 反应体系的吸光度差异，提出了一种方便、灵敏的 TAC 测定方法。在最佳条件下，该比色传感器的检测限（LOD）为1.17 μM，线性浓度范围为1 μM~30 μM。分析结果表明，该比色传感器在果蔬食品中的实际应用具有良好的可行性，为比色生物传感器的建立提供了新的途径。
The total antioxidant capacity (TAC) has become increasingly vital for evaluating antioxidant food quality in the field of healthcare. Herein, a convenient and sensitive method for TAC assay was proposed based on the absorbance difference of reaction systems between various antioxidants existed in food and Dex-FeMnzyme/ oxTMB. Under the optimum condition, the limit of detection (LOD) of the colorimetric sensor was 1.17 mu M with the linear concentration range from 1 mu M to 30 mu M. The analysis results demonstrated the excellent feasibility of practical application in fruit and vegetable food, which offered a new avenue for the establishment of colorimetric biosensors.