Apigenin attenuates patulin-induced apoptosis in HEK293 cells by modulating ROS-mediated mitochondrial dysfunction and caspase signal pathway
Apigenin attenuates patulin-induced apoptosis in HEK293 cells by modulating ROS-mediated mitochondrial dysfunction and caspase signal pathway
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芹菜素通过调节 ROS 介导的线粒体功能障碍和 caspase 信号通路来减弱展曲霉素诱导的 HEK293 细胞凋亡
DOI：
10.1016/j.toxicon.2017.07.018
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发表时间：
2017-10-01
期刊：
影响因子：
2.8
通讯作者：
Peng, Xiaoli
中科院分区：
文献类型：
--
作者：
Zhong, Yujie;Jin, Chengni;Peng, Xiaoli
霉菌毒素如棒曲霉素（PAT）是对公众健康最重要的食品污染物之一。本研究旨在评价植物源性食品中最具生物活性的黄酮类化合物之一芹菜素（API）对PAT诱导的HEK 293细胞凋亡的保护作用。在碱性条件下，用8 μ M PAT不加或加API（2.5、5和10 μ M）处理细胞10小时。API通过抑制细胞内活性氧（ROS）的积累，调节氧化磷酸化尤其是ATP合成酶的表达，重建线粒体膜电位（MMP），维持较高的细胞内ATP水平，同时下调p53、Bax和上调Bcl-2的表达，发挥肾脏保护作用。因此，细胞色素c从线粒体向细胞质的释放减少，导致引发剂半胱天冬酶-9和执行剂半胱天冬酶（3、6和7）表达和酶活性的抑制。结果显示，芹菜素通过调节ROS介导的线粒体功能障碍和caspase信号通路，减轻展青霉素诱导的HEK 293细胞凋亡。2017由Elsevier Ltd.出版
Mycotoxins like patulin (PAT) are among the most significant food contaminant with regard to public health. This study aimed to evaluate the protective effect of apigenin (API), one of the most bioactive flavonoids in plant-derived food, on PAT-induced apoptosis in HEK293 cells. Cells were treated under basic conditions, 8 mu M PAT without or with API (2.5, 5 and 10 mu M) concomitantly for 10 h. API exerted renoprotective effect by inhibiting intracellular reactive oxygen species (ROS) accumulation, modulating oxidative phosphorylation especially elevating the expression of ATP synthase, re-establishing mitochondrial membrane potential (MMP) and maintaining higher intracellular ATP level, accompanied by p53, Bax downregulation and Bcl-2 upregulation. Thereby, cytochrome c release from mitochondria to cytoplasm was reduced, causing inhibition of initiator caspases-9 and executioner caspases (3, 6 and 7) expression and enzyme activities. Results revealed dietary apigenin attenuates patulin-induced apoptosis in HEK293 cells by modulating ROS-mediated mitochondrial dysfunction and caspase signal pathway. 2017 Published by Elsevier Ltd.