POSSIBLE INVOLVEMENT OF 1-ALPHA,25-DIHYDROXYVITAMINE D3 IN PROLIFERATION AND DIFFERENTIATION OF 3T3-L1 CELLS
POSSIBLE INVOLVEMENT OF 1-ALPHA,25-DIHYDROXYVITAMINE D3 IN PROLIFERATION AND DIFFERENTIATION OF 3T3-L1 CELLS
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DOI：
10.1016/s0006-291x(88)80482-0
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发表时间：
1988-03-30
影响因子：
3.1
通讯作者：
BABA, S
中科院分区：
文献类型：
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作者：
ISHIDA, Y;TANIGUCHI, H;BABA, S
10-10-10-7M的1，α，25-二羟基维生素D3[1，α，25(OH)2D3]以剂量和时间依赖的方式抑制3T3-L1细胞的生长。1α，25(OH)2D3诱导3T3-L1细胞分化的能力较低，因为1α，25(OH)2D3并未诱导成成熟的脂肪细胞样细胞，而是转变为胞浆内含有小滴状物质的稍圆的细胞，分化为脂肪细胞的标志酶甘油磷酸脱氢酶(sn-甘油-3-磷酸：NAD+2-氧化还原酶，EC 1.1.1.8)没有增加。这些结果以及这种维生素的自然存在表明，除了肠道钙转运和骨矿动员等已知作用外，1α，25(OH)2D3可能在细胞生长和分化中发挥重要作用。
Growth of 3T3-L1 cells was inhibited by 10-10-10-7 M of 1.alpha.,25-dihydroxy vitamine D3 [1.alpha.,25(OH)2D3] in a dose- and time-dependent manner. The potency of 1.alpha.,25(OH)2D3 in inducing differentiation was low, since 3T3-L1 cells cultured with 1.alpha.,25(OH)2D3 did not become mature adipocyte-like cells but were changed to slightly rounded cells containing small droplet-like substances in the cytoplasm and glycerophosphate dehydrogenase (sn-glycerol-3-phosphate: NAD+2-oxidoreductase, EC 1.1.1.8), the marker enzyme of differentiation to adipocyte, did not increase. These results together with the natural occurrence of this vitamin indicate that 1.alpha.,25(OH)2D3 may play an important role in the cell growth and differentiation besides such known action as intestinal calcium transport and bone mineral mobilization.