Comparison of germinal center markers CD10, BCL6 and human germinal center-associated lymphoma (HGAL) in follicular lymphomas
Comparison of germinal center markers CD10, BCL6 and human germinal center-associated lymphoma (HGAL) in follicular lymphomas
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DOI：
10.1186/1746-1596-6-97
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发表时间：
2011-10-11
影响因子：
2.6
通讯作者：
Leoni, Pietro
中科院分区：
文献类型：
--
作者：
Goteri, Gaia;Lucarini, Guendalina;Leoni, Pietro
背景：近期，人生发中心相关淋巴瘤（HGAL）基因蛋白已被提议作为CD10和BCL6的一种辅助滤泡标记物。
方法：我们的目的是评估82例滤泡性淋巴瘤（FLs）中HGAL的免疫反应性，其中67例为淋巴结型，5例为皮肤型，10例为转化型，所有病例均通过组织学、免疫组织化学和聚合酶链反应（PCR）进行分析。
结果：在FLs中，HGAL免疫染色阳性率（97.6%）高于BCL6（92.7%）和CD10（90.2%）；bcl6和/或CD10阴性的病例HGAL均为阳性，而HGAL阴性的2例BCL6为阳性；在不同恶性亚型或级别之间未发现HGAL免疫染色的差异。
结论：因此，HGAL可作为最敏感的生发中心（GC）标记物用于FLs的免疫染色；单独使用时，可将免疫染色成本降低一半，仅在HGAL阴性病例中使用其他两种标记物。
Background: Recently, human germinal center-associated lymphoma (HGAL) gene protein has been proposed as an adjunctive follicular marker to CD10 and BCL6.Methods: Our aim was to evaluate immunoreactivity for HGAL in 82 cases of follicular lymphomas (FLs) - 67 nodal, 5 cutaneous and 10 transformed - which were all analysed histologically, by immunohistochemistry and PCR.Results: Immunostaining for HGAL was more frequently positive (97.6%) than that for BCL6 (92.7%) and CD10 (90.2%) in FLs; the cases negative for bcl6 and/or for CD10 were all positive for HGAL, whereas the two cases negative for HGAL were positive with BCL6; no difference in HGAL immunostaining was found among different malignant subtypes or grades.Conclusions: Therefore, HGAL can be used in the immunostaining of FLs as the most sensitive germinal center (GC)-marker; when applied alone, it would half the immunostaining costs, reserving the use of the other two markers only to HGAL-negative cases.