PATHOGENICITY OF FUSARIUM PROLIFERATUM, A NEW CAUSAL AGENT OF POKKAH BOENG IN SUGARCANE
PATHOGENICITY OF FUSARIUM PROLIFERATUM, A NEW CAUSAL AGENT OF POKKAH BOENG IN SUGARCANE
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发表时间：
2013
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通讯作者：
K. Khani;A. Alizadeh;R. F. Nejad;A. Tehrani
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作者：
K. Khani;A. Alizadeh;R. F. Nejad;A. Tehrani
Pokkah Boeng是爪哇语中的一个术语，表示畸形或扭曲的顶部。刀伤症状也与Pokkah Boeng有关。在1993-2001年间，从受感染的茎上采集了样本，这些茎在生长点附近出现梯状病变。所有样品都在自来水中彻底清洗，并用70%乙醇消毒。用蒸馏水冲洗后，将小块感染组织接种于改良的Nash&Snyder和马铃薯葡萄糖琼脂(PDA)培养基上，在25℃下培养10~14d。采用PDA、康乃馨叶片琼脂(CLA)、蔗糖营养琼脂(SNA)和KCl琼脂培养基对菌株进行鉴定。他们将四种镰刀菌归类为串珠镰刀菌(F.moniliforme)。增殖镰刀菌F.亚粘菌素和半乳杆菌分别占55%、21.5%、17.6%和5.9%。对这些真菌的致病力测定表明，除半乳镰刀菌外，其余菌株对甘蔗均有致病作用。在致病菌株中，增殖镰刀菌的致病性低于黄萎病菌和亚粘性镰刀菌。该菌对甘蔗的致病性在世界上尚属首次报道。
POKKAH BOENG IS A JAVANESE term denoting a malformed or distorted top. A knife-cut symptom is also associated with pokkah boeng. During 1993–2001, samples were collected from infected stalks showing ladder-like lesions near the growing point. All samples were thoroughly washed in tap water and disinfected with 70% ethanol. After rinsing with distilled water, small pieces of infected tissues were plated on modified Nash & Snyder and potato dextrose agar (PDA) media and incubated at 25 °C for 10 to 14 days. PDA, carnation leaf agar (CLA), sucrose nutrient agar (SNA) and KCl agar media were used for identification of isolates. They categorised four Fusarium species identified as Fusarium verticillioides (F. moniliforme). F. proliferatum. F. subglutinans and F. semitectum with 55%, 21.5%, 17.6% and 5.9% frequency, respectively. Pathogenicity studies with these fungi indicated that all isolates were pathogenic to sugarcane except isolates of F. semitectum. Among pathogenic isolates, F. proliferatum was less pathogenic than F. verticillioides and F. subglutinans. This is the first report of the pathogenicity of F. proliferatum on sugarcane in the world.