Evaluating the levels of CSF and serum factors in ALS
Evaluating the levels of CSF and serum factors in ALS
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DOI：
10.1002/brb3.637
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发表时间：
2017-03-01
影响因子：
3.1
通讯作者：
Zang, Dawei
中科院分区：
文献类型：
--
作者：
Guo, Jie;Yang, Xuan;Zang, Dawei
目的：本研究的目的是确定CSF和血清因子作为生物标志物，可能有助于区分ALS患者和对照组，并预测ALS的进展和预后。方法：采用ELISA法对105例ALS患者和56例对照者的血清和脑脊液进行13项因素分析。采用修订后的ALS功能评定量表（ALSFRS-r）评估ALS患者的整体功能状态，并在入组后对ALS患者进行了5年的电话或门诊随访。最后，我们通过多变量统计模型检验因子水平与各种临床参数之间的相关性，并评估其对预后的预测价值。结果：脑脊液中8项指标明显升高，血清中12项指标明显升高。相关分析显示，ALS患者bFGF、VEGF、MIP-1水平升高，病程延长，CSF和血清中疾病进展缓慢。MCP-1水平升高与疾病严重程度加重和进展加快相关，CSF或血清中IFN-水平与疾病进展呈正相关。最后，脑脊液中bFGF、脑脊液和血清中VEGF水平升高，预后较好；相反，脑脊液中IFN-水平较高的患者总生存期较短。结论：我们证明了ALS患者的因子谱与对照组不同，可能在临床实践和治疗试验中有用。
Objectives: The aim of this study was to identify CSF and serum factors as biomarkers that may aid in distinguishing ALS patients from control subjects and predicting ALS progression as well as prognosis.Methods: Serum and CSF samples from 105 patients with ALS and 56 control subjects were analyzed for 13 factors using ELISA. The revised ALS functional rating scale (ALSFRS-r) was used to evaluate the overall functional status of ALS patients, and we also followed up with ALS patients either by phone or with clinic visits for five years after enrollment in this study. Finally, we examined the correlations between factor levels and various clinical parameters and evaluated the predictive value for prognosis through a multivariate statistic model.Results: A total of eight factors were obviously elevated in CSF, and twelve markers were increased in serum. In the correlation analyses, there were trends toward higher bFGF, VEGF, MIP-1 levels in ALS with a longer disease duration and slower disease progression in both CSF and serum. Higher MCP-1 levels were associated with worse disease severity and faster progression, and the IFN- levels were positively associated with disease progression in either CSF or serum. Finally, a better prognosis was observed with higher levels bFGF in CSF and VEGF in CSF and serum; conversely, patients with higher levels of IFN- in the CSF had shorter overall survival.Conclusions: We demonstrated that a factor profile of ALS patients is distinct from control subjects and may be useful in clinical practice and therapeutic trials.