Optimal conditions for peripheral nerve storage in green tea polyphenol: an experimental study in animals
Optimal conditions for peripheral nerve storage in green tea polyphenol: an experimental study in animals
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DOI：
10.1016/j.jneumeth.2005.01.011
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发表时间：
2005-06-30
影响因子：
3
通讯作者：
Nakamura, T
中科院分区：
文献类型：
--
作者：
Matsumoto, T;Kakinoki, R;Nakamura, T
我们以前的研究表明，成功的周围神经存储1个月使用多酚溶液。我们在这里报告了两项研究，以解决残留问题，使用多酚作为周围神经的存储解决方案。研究1旨在确定多酚溶液的最佳浓度和神经储存的最佳浸泡时间。将大鼠坐骨神经段浸入三种不同浓度（2.5，1.0和0.5 mg/ml）的多酚溶液中，持续三个不同的时间（1，7和26天）。电生理学和形态学研究表明，神经再生的神经节段，已浸泡在1毫克/毫升的多酚溶液1周，并在Dulbecco的改良的Eagle培养基（DMEM）为随后的3周是上级其他治疗组的再生。在研究2中，使用在1.0 mg/ml多酚溶液中储存1周和随后在DMEM中储存3周的犬坐骨神经节段研究神经组织对多酚溶液的渗透性。电镜观察发现，神经束膜500-700 μ m范围内的雪旺细胞结构保存完好，但500-700 μ m范围内的细胞严重受损或消失。推测多酚溶液渗入神经组织的限度为500-700 μ m。(c)2005 Elsevier B. V.保留所有权利。
Our previous study demonstrated successful peripheral nerve storage for 1 month using polyphenol solution. We here report two studies to solve residual problems in using polyphenols as a storage solution for peripheral nerves. Study 1 was designed to determine the optimal concentration of the polyphenol solution and the optimal immersion period for nerve storage. Rat sciatic nerve segments were immersed in polyphenol solution at three different concentrations (2.5, 1.0, and 0.5 mg/ml) for three different periods( 1, 7, and 26 days). Electrophysiological and morphological studies demonstrated that nerve regeneration from nerve segments that had been immersed in 1 mg/ml polyphenol solution for 1 week and in Dulbecco's modified Eagle's medium (DMEM) for the subsequent 3 weeks was superior to the regeneration in other treatment groups. In study 2, the permeability of nerve tissue to polyphenol solution was investigated using canine sciatic nerve segments stored in 1.0 mg/ml polyphenol solution for 1 week and in DMEM for the subsequent 3 weeks. Electron microscopy revealed that the Schwann cell structure within 500-700 mu m of the perineurium was preserved, but cells deeper than 500-700 mu m were badly damaged or had disappeared. The infiltration limit for polyphenol solution into neural tissue is inferred to be 500-700 mu m. (c) 2005 Elsevier B.V. All rights reserved.