Direct electron transfer and electrocatalysis of hemoglobin immobilized on graphene-Pt nanocomposite
Direct electron transfer and electrocatalysis of hemoglobin immobilized on graphene-Pt nanocomposite
复制标题
石墨烯-Pt纳米复合材料固定化血红蛋白的直接电子转移和电催化
DOI：
10.1016/j.jelechem.2011.03.004
复制
发表时间：
2011-07-01
影响因子：
4.5
通讯作者：
Hou, Wenhua
中科院分区：
文献类型：
--
作者：
Feng, Xiaomiao;Li, Ruimei;Hou, Wenhua
通过在石墨烯-铂纳米复合材料上吸附血红蛋白（Hb），制备了一种新型的Hb电极。采用微波辅助法制备了具有均匀片状结构的石墨烯-铂。Soret吸收带的位置表明lib在吸附到石墨烯-Pt上后保持其天然构型。用循环伏安法研究了固定化血红蛋白的电化学行为。固定化血红蛋白在-0.26V的形式电位处有一对稳定的峰，峰形清晰。以此为模型，研制了一种新型的第三代H2 O2生物传感器。该电极对H2 O2的还原具有良好的电催化性能，稳定性好，干扰小，重现性好。石墨烯的存在增强了蛋白质的负载，加速了界面电子转移，并提高了吸附蛋白质的热稳定性。(C)2011爱思唯尔有限公司版权所有。
A novel hemoglobin (Hb) electrode has been presented through adsorbing lib onto graphene-Pt nanocomposite. The graphene-Pt with uniform sheet-like structure was obtained by microwave-assisted method. Positions of Soret absorption band showed lib retained its natural configuration after adsorption onto graphene-Pt. The electrochemical behavior of the immobilized Hb was studied by cyclic voltammetry. The immobilized Hb showed a pair of stable and well-defined peaks at the formal potential of -0.26 V. As a model, a kind of novel third generation biosensor for H(2)O(2) was developed. The resultant heme-protein electrode exhibited electrocatalytical responses to the reduction of H(2)O(2), excellent stability, interference and good reproducibility. The presence of graphene enhanced the protein loadings, accelerates interfacial electron transfer and improves thermal stability of the adsorbed protein. (C) 2011 Elsevier B.V. All rights reserved.