IGA ANTIBODY-PRODUCING CELLS IN PERIPHERAL-BLOOD AFTER ANTIGEN INGESTION - EVIDENCE FOR A COMMON MUCOSAL IMMUNE-SYSTEM IN HUMANS
IGA ANTIBODY-PRODUCING CELLS IN PERIPHERAL-BLOOD AFTER ANTIGEN INGESTION - EVIDENCE FOR A COMMON MUCOSAL IMMUNE-SYSTEM IN HUMANS
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DOI：
10.1073/pnas.84.8.2449
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发表时间：
1987-04-01
影响因子：
11.1
通讯作者：
MESTECKY, J
中科院分区：
文献类型：
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作者：
CZERKINSKY, C;PRINCE, SJ;MESTECKY, J
摄入抗原导致外分泌物中伊加抗体的选择性诱导，这一发现表明抗原使派伊尔淋巴结淋巴细胞敏感，这些淋巴细胞迁移到粘膜部位并产生局部分泌型伊加（S-IgA）抗体应答。由于很难证明派伊尔氏斑细胞的迁移行为，因此关于人类共同粘膜免疫系统的证据很少。在本研究中，外周血单个核细胞（PBMC）从人类志愿者谁摄入胶囊含有杀死链球菌突变体进行了测定自发抗体产生细胞。5名志愿者中有4名在7天内表现出循环IgA产生细胞，并在第10-12天达到最大反应。1例IgA缺陷受试者表现出动力学相同的IgM应答。美洲商陆促分裂原刺激PBMC产生抗S。主要是伊加同种型的变形抗体。显著的S-IgA抗S。到第14天，在唾液和泪液中检测到突变体抗体，并且抗体在3周时达到最大滴度。血清抗S无变化。观察到变异抗体。IgA缺陷受试者产生唾液分泌型IgM抗体。这些结果表明，抗原摄入后，外周血中含有抗原特异性的伊加浆细胞的前体，它们的存在先于外部分泌物中的S-IgA抗体的出现。因此，这些实验进一步支持了人类存在共同的粘膜免疫系统。
The findings that ingestion of antigens results in the selective induction of IgA antibodies in external secretions suggests that antigen sensitizes Peyer''s patch lymphoid cells, which migrate to mucosal sites and generated local secretory IgA (S-IgA) antibody responses. Evidence for a common mucosal immune system in humans has been scanty because of the difficulty in demonstrating migratory behavior of Peyer''s patch cells. In the present study, peripheral blood mononuclear cells (PBMC) from human volunteers who had ingested capsules containing killed Streptococcus mutants were assayed for spontaneous antibody-producing cells. Four of five volunteers exhibited circulating IgA-producing cells within 7 days and reached maximum responses by days 10-12. One IgA-deficient subject exhibited IgM responses with identical kinetics. Pokeweed-mitogen-stimulated PBMC produced anti-S. mutans antibodies predominantly of the IgA isotype. Significant S-IgA anti-S. mutants antibodies were detected in saliva and tears by day 14, and the antibodies reached maximum titers by 3 weeks. No changes in serum anti-S. mutans antibodies were noted. The IgA-deficient subject produced salivary secretory IgM antibodies. These results suggest that, after antigen ingestion, peripheral blood contains antigen-specific precursors of IgA plasma cells and that their presence precedes the appearance of S-IgA antibodies in external secretions. Therefore, these experiments provide further support for the existence of a common mucosal immune system in humans.