Modulatory Effects of Lycopene and Ellagic Acid on Reproductive Dysfunction Induced by Polychlorinated Biphenyl (Aroclor 1254) in Male Rats
Modulatory Effects of Lycopene and Ellagic Acid on Reproductive Dysfunction Induced by Polychlorinated Biphenyl (Aroclor 1254) in Male Rats
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DOI：
10.1111/j.1742-7843.2009.00529.x
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发表时间：
2010-06-01
影响因子：
3.1
通讯作者：
Ceribasi, Ali Osman
中科院分区：
文献类型：
--
作者：
Atessahin, Ahmet;Turk, Gaffari;Ceribasi, Ali Osman
本研究旨在探讨番茄红素(LP)和鞣花酸(EA)对Aroclor(AR)1254所致雄性大鼠睾丸和精子氧化应激和细胞凋亡毒性的可能保护作用。对照组给予安慰剂治疗。LP(10 mg/kg/隔日)、EA(2 mg/kg/隔日)和AR(2 mg/kg/d)组分别单独给予LP、EA和AR。后两组一组接受AR+LP治疗，另一组接受AR+EA治疗。观察大鼠体重、生殖器官重量、附睾精子特性、睾丸组织脂质过氧化水平、抗氧化酶活性、组织病理学改变及Bax和Bcl2基因诱导的细胞凋亡情况。AR染毒后，大鼠体重、附睾精子密度、睾丸超氧化物歧化酶活性、曲细精管直径、生精细胞层厚度和Johnsen‘s评分均显著降低，睾丸、附睾膜和精泡的相对重量、精子畸形率和细胞凋亡率显著升高，生精细胞变性、脱落、排列紊乱，睾丸组织间质水肿充血。与单纯AR组比较，AR组大鼠的精子畸形率、睾丸硫代巴比妥酸反应物质含量显著降低，谷胱甘肽(GSH)含量、GSH-过氧化物酶活性、过氧化氢酶活性和附属膜精子密度显著升高。此外，LP和EA分别可完全或部分恢复AR所致的组织病理损伤。AR通过破坏氧化剂/抗氧化剂的平衡和增加生精细胞的凋亡率来损害睾丸组织和精子。但LP和EA对AR所致雄性大鼠生殖功能障碍均有调节作用。
The present study was conducted to investigate the possible protective effects of lycopene (LP) and ellagic acid (EA) on aroclor (AR) 1254-induced testicular and spermatozoal toxicity associated with the oxidative stress and apoptosis in male rats. The control group was treated with placebo. LP (10 mg/kg/every other day), EA (2 mg/kg/every other day) and AR (2 mg/kg/day) groups were given alone LP, EA and AR respectively. One of the last two groups received AR + LP, and the other treated with AR + EA. Body and reproductive organ weights, epididymal sperm characteristics, testicular tissue lipid peroxidation levels, antioxidant enzyme activities, histopathological changes and apoptosis via Bax and Bcl-2 genes were investigated. AR administration caused statistically significant decreases in body-weight, epididymal sperm concentration, testicular superoxide dismutase activity, diameters of seminiferous tubules, germinal cell layer thickness and Johnsen's testicular score, and increases in relative weights of testis, epidydimis and seminal vesicles, rates of abnormal sperm and apoptotic cell expression along with degeneration, desquamation and disorganization in spermatogenic cells, and interstitial oedema and congestion in testicular tissue. LP and EA treatments to AR-treated rats markedly decreased abnormal sperm rates, testicular thiobarbituric acid reactive substances level, and increased the glutathione (GSH) level, GSH-peroxidase, catalase activities and epidiymal sperm concentration as compared with the alone AR group. Additionally, the AR-induced histopathological damages were totally or partially recovered by LP or EA administrations respectively. AR damages the testicular tissue and spermatozoa by impairing the oxidant/antioxidant balance and by increasing the apoptotic spermatogenic cell rates. However, both LP and EA have modulator effects on AR-induced reproductive dysfunction in male rats.