Interaction of nominally soluble proteins with phospholipid monolayers at the air-water interface
Interaction of nominally soluble proteins with phospholipid monolayers at the air-water interface
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DOI：
10.1016/s0005-2736(02)00405-4
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发表时间：
2002-08-19
影响因子：
3.4
通讯作者：
Huestis, WH
中科院分区：
文献类型：
--
作者：
Pitcher, WH;Keller, SL;Huestis, WH
在生理pH和离子强度下，研究了一氧化碳血红蛋白（HbCO）、甘油醛-3-磷酸脱氢酶（GAPDH）和聚组氨酸与磷脂单分子膜在空气-水界面上的相互作用。HbCO和GAPDH都与含有带负电荷的脂质的单层更强烈地相互作用。HbCO和GAPDH与脂质单层的相互作用随着pH值的增加而降低。HbCO-单层和GAPDH-单层的相互作用可以建模为扩散限制过程，动力学数据拟合拉伸指数方程。这些动力学的意义进行了讨论。聚组氨酸仅与含有带负电荷头部基团的脂质的单层相互作用。总之，所呈现的结果与具有大静电组分的UbCO-脂质相互作用、具有可比静电和疏水组分的GAPDH-脂质相互作用以及仅为静电的聚组氨酸-脂质相互作用一致。(C)2002 Elsevier Science B. V.保留所有权利。
The interactions of carbonmonoxyhemoglobin (HbCO), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and polyhistidine with phospholipid monolayers at the air-water interface were studied at physiological pH and ionic strength. HbCO and GAPDH both interact more strongly with monolayers containing negatively charged lipids. The interaction of HbCO and GAPDH with lipid monolayers decreases with increasing pH. Both the HbCO-monolayer and the GAPDH-monolayer interactions can be modeled as diffusion-limited processes, with kinetic data fit to a stretched exponential equation. The significance of these kinetics are discussed. Polyhistidine interacts only with monolayers containing lipids with negatively charged headgroups. In total, the results presented are consistent with an UbCO-lipid interaction with a large electrostatic component, a GAPDH-lipid interaction with comparable electrostatic and hydrophobic components, and a polyhistidine-lipid interaction that is solely electrostatic. (C) 2002 Elsevier Science B.V. All rights reserved.