Lack of inhibition by atrial natriuretic factor on cyclic AMP levels in single nephron segments and the glomerulus.
Lack of inhibition by atrial natriuretic factor on cyclic AMP levels in single nephron segments and the glomerulus.
复制标题
心钠素对单个肾单位节段和肾小球中环磷酸腺苷水平缺乏抑制作用。
DOI：
10.1016/s0006-291x(85)80035-8
复制
发表时间：
1985
影响因子：
3.1
通讯作者：
Pettinger,WA
中科院分区：
文献类型：
--
作者：
Umemura,S;Smyth,DD;Pettinger,WA
近年来，心钠素（ANF）对血管组织腺苷酸环化酶系统的抑制作用已被报道。在寻求类似的影响，在肾组织中，我们研究了心钠素对环磷酸腺苷水平在单个肾单位节段和肾小球的大鼠。将单个肾单位节段或肾小球在磷酸二酯酶抑制剂存在下孵育2分钟，加入或不加入甲状旁腺激素（PTH）或精氨酸加压素（AVP）和不同浓度的ANF，37°C。在近曲小管、皮质集合小管和肾小球中证明了α2-肾上腺素受体抑制腺苷酸环化酶的能力。然而，心钠素不能抑制cAMP的形成，在任何这些肾单位节段，也不是在肾小球。因此，与血管系统不同，ANF对这些肾组织中cAMP的形成没有抑制作用。
Recently an inhibitory effect of atrial natriuretic factor (ANF) on the adenylate cyclase system has been reported in vascular tissue. In seeking similar affects in renal tissue, we studied the effect of ANF on cyclic AMP levels in single nephron segments and in glomeruli from the rat. Individual nephron segments or glomeruli were incubated in the presence of a phosphodiesterase inhibitor, with or without parathyroid hormone (PTH) or arginine vasopressin (AVP) and varying concentrations of ANF at 37°C for 2 min. The capacity for α2-adrenoceptor inhibition of adenylate cyclase was demonstrated in the proximal convoluted tubule, cortical collecting tubule and in glomeruli. Nevertheless, ANF could not inhibit cAMP formation in any of these nephron segments nor in the glomerulus. Thus, unlike the vasculature, ANF has no inhibitory effect on cAMP formation in these renal tissues.