Effect of triazole pesticide formulation on bovine culture cells
Effect of triazole pesticide formulation on bovine culture cells
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DOI：
10.1080/03601234.2013.824224
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发表时间：
2013-12-02
影响因子：
2
通讯作者：
Galdikova, Martina
中科院分区：
文献类型：
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作者：
Holeckova, Beata;Sivikova, Katarina;Galdikova, Martina
迄今为止，有关三唑类农药可能产生的基因毒性作用的大多数数据都集中在实验动物上，导致有关牛等其他非目标生物体的信息有限。本研究的目的是研究三唑（戊唑醇/丙硫菌唑）杀菌剂制剂对诱导牛培养淋巴细胞中染色体畸变（CA）、姐妹染色单体交换（SCE）和DNA片段化的影响。我们的结果表明，处理 24 小时后，杀菌剂制剂并未在牛细胞中诱导大量 CA。尽管如此，观察到有丝分裂的剂量依赖性减少，在两个供体中在30.0g mL(-1)时效果最强（分别为P < 0.01和P < 0.001）。延长48小时暴露导致处理培养物中断裂水平增加(3.0-15.0g mL(-1); P < 0.05)和有丝分裂指数(MI)显着降低。所测试的杀菌剂在处理 24 小时和 48 小时后均未对 SCE 频率产生任何统计变化。然而，24 小时后观察到增殖指数 (PI) 显着下降，表明杀菌剂对细胞周期动力学的影响。长时间暴露48小时会导致细胞毒性，主要在最高杀菌剂浓度（30.0g·mL(-1)，P < 0.001）下相对于对照表现出较低的PI值。使用牛 1、5 和 7 号染色体（BTA1、BTA5 和 BTA7）的涂色探针仅检测到低水平的非整倍体。 3.0g·mL(-1)剂量的杀菌剂48h后诱导多倍体细胞显着增加(P<0.05)。 DNA片段化分析没有揭示细胞培养物中任何时间（24小时和48小时）DNA核小体梯的存在以及杀菌剂浓度。
To date, most data about the possible genotoxic effect of triazole pesticides are focused on laboratory animals resulting in limited information on further non-target organisms such as cattle. The objective of the present study was to investigate the effect of triazole (tebuconazole/prothioconazole) fungicide formulation on the induction of chromosomal aberrations (CAs), sister chromatid exchanges (SCEs) and DNA fragmentation in bovine cultured lymphocytes. Our results showed that the fungicide formulation did not induce significant number of CAs in bovine cells after 24h treatment. Nevertheless, the dose-dependent reduction of mitotic division was observed, with the strongest effect at 30.0g mL(-1) in both donors (P < 0.01 and P < 0.001, respectively). Prolonged 48h exposure caused the increased level of breaks in treated cultures (3.0-15.0g mL(-1); P < 0.05) and significant decrease in mitotic index (MI). The tested fungicide failed to produce any statistical changes in the SCE frequency neither after 24h nor 48h treatment. However, the significant decline of the proliferation index (PI) was observed after 24h indicating the fungicide influence on cell cycle kinetics. Prolonged 48h exposure caused cytotoxicity reflecting in lower PI value relative to control mainly at the highest fungicide concentrations (30.0g mL(-1), P < 0.001). Using painting probes for bovine chromosomes 1, 5 and 7 (BTA1, BTA5 and BTA7) only low levels of aneuploidies were detected. Significant increase of polyploidy cells (P < 0.05) was induced by a 3.0g mL(-1) dose of the fungicide after 48h. DNA fragmentation assay didn't reveal the presence of DNA nucleosome ladder in cell cultures at any time (24h and 48h) and fungicide concentration.