BACTERIOPHAGE INFECTION . WHICH END OF SP82G GENOME GOES IN FIRST
BACTERIOPHAGE INFECTION . WHICH END OF SP82G GENOME GOES IN FIRST
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DOI：
10.1128/jvi.5.2.194-198.1970
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发表时间：
1970-01-01
影响因子：
5.4
通讯作者：
MCALLISTER, WT
中科院分区：
文献类型：
--
作者：
MCALLISTER, WT
脱氧核糖核酸（DNA）从噬菌体SP82G向其宿主的转移可被冷却所阻止，但氯霉素、放线菌素D或氰化物对其影响很小。标记物进入噬菌体基因组的顺序通过间隔地停止DNA的转移，通过混合去除未转移的DNA，并测定混合复合物中标记物的存在来确定。噬菌体基因组上的标记以线性、极性方式转移，与先前确定的遗传和物理图谱一致。那些与早期功能有关的标记物首先进入，并且转移速率是温度依赖性的。
The transfer of deoxyribonucleic acid (DNA) from bacteriophage SP82G to its host may be halted by chilling but is affected little by chloramphenicol, actinomycin D, or cyanide. The order of entry of markers on the phage genome was determined by halting the transfer of DNA at intervals, removing the untransferred DNA by blending, and assaying for the presence of markers in the blended complexes. Markers on the phage genome are transferred in a linear, polar fashion consistent with the previously determined genetic and physical maps. Those markers concerned with early functions enter first, and the rate of transfer is temperature dependent.