Gene expression of transforming growth factor-β receptors types I and II in rat endometrium during the estrous cycle and early pregnancy
Gene expression of transforming growth factor-β receptors types I and II in rat endometrium during the estrous cycle and early pregnancy
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DOI：
10.1016/j.lfs.2005.10.012
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发表时间：
2006-05-01
期刊：
影响因子：
6.1
通讯作者：
Zhu, C
中科院分区：
文献类型：
--
作者：
Lin, HY;Qian, D;Zhu, C
转化生长因子-β受体I型(TβRI)和II型(TβRII)在啮齿动物动情周期和着床中的作用目前尚不清楚。本研究采用原位杂交和半定量逆转录聚合酶链式反应(RT-PCR)技术，检测了大鼠动情周期、着床前和围着床期子宫内膜中TβRI和TβRII的时空表达。原位杂交显示，在动情周期中，TβRI和TβRII在大鼠子宫内膜中有微弱表达。在着床前，这两种受体在妊娠0.5天和1.5天的腔上皮和腺上皮中均有表达，但在2.5天和3.5天表达下调。在围着床期，两种转化生长因子-β受体均定位于子宫腔上皮和上皮下基质，以促进附着反应和滋养细胞的侵袭。它们在第4.5天高表达，而在第5.5天和6.5天表达下调。半定量RT-PCR分析证实了原位杂交获得的数据。这些结果表明，在着床前，两种转化生长因子-β受体在子宫内膜上皮细胞的增殖中起作用。在围着床期，这两种转化生长因子-β受体在子宫容受性和依附反应的启动过程中起着重要作用。当滋养层细胞开始入侵时，转化生长因子-β信号被下调。(C)2005 Elsevier Inc.保留所有权利。
Roles of transforming growth factor-beta (TGF-beta) receptor types I (T beta RI) and II (T beta RII) during the estrous cycle and implantation of rodents are currently unclear. In the present study, the spatial and temporal expressions of T beta RI and T beta RII in rat endometrium during the estrous cycle, pre-, and peri-implantation were examined using in situ hybridization and semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). With in situ hybridization, T beta RI and T beta RII were expressed at weak levels in rat endometrium during the estrous cycle. During pre-implantation, both receptors were expressed in the luminal epithelium and glandular epithelium on Days 0.5 and 1.5 of pregnancy, but were down-regulated on Days 2.5 and 3.5. During peri-implantation, both TGF-beta receptors were localized in the luminal epithelium and subepithelial stroma to facilitate attachment reaction and trophoblast invasion. They were highly expressed on Day 4.5, whereas were down-regulated on Days 5.5 and 6.5. Semi-quantitative RT-PCR analysis confirmed the data obtained by in situ hybridization. These results suggest that during pre-implantation, both TGF-beta receptors are functional in the proliferation of endometrial epithelial cells. During peri-implantation, both TGF-beta receptors play important roles during the onset of the uterine receptivity and the attachment reaction. TGF-beta signaling is down-regulated when trophoblast invasion begins. (c) 2005 Elsevier Inc. All rights reserved.