Caspase-dependent retinal ganglion cell apoptosis in the rat model of acute diabetes
Caspase-dependent retinal ganglion cell apoptosis in the rat model of acute diabetes
复制标题
急性糖尿病大鼠模型中Caspase依赖性视网膜神经节细胞凋亡
DOI：
10.1097/00029330-200812020-00018
复制
发表时间：
2008-12-20
影响因子：
6.1
通讯作者：
Wang Xiang-gui
中科院分区：
文献类型：
--
作者：
Li Yong-hao;Zhuo Ye-hong;Wang Xiang-gui
Background Neural apoptosis is generally believed to be mediated by two distinct pathways, caspase-dependant and caspase-independent pathways. This study investigated the apoptotic pathways involved in retinal ganglion cells in acute diabetes in rats.Methods Diabetes was induced in male Wistar rats by a peritoneal injection of streptozotocin (STZ). Expression and localization of caspase-3 and apoptosis-inducing factor (AIF) proteins in the retina of diabetic rats was examined by Western blotting and immunohistochemistry analyses. Terminal transferase dUTP nick end labeling (TUNEL) assay and immunofluorescent staining specific for caspase-3 and AIF were applied to analyze for apoptosis of retinal ganglion cells. In addition, a caspase-3 inhibitor DEVD-CHO was injected intravitreally to further determine the apoptotic pathways of retinal ganglion cells triggered in acute diabetes.Results Two weeks after induction of diabetes, a significant increase in caspase-3 protein expression and localization occurred in the nerve fiber layer, ganglion cell layer, and inner plexiform layer of the retina. Four weeks after the onset of diabetes, the increase in caspase-3 expression was profound eight weeks postinduction of diabetes (P
