Expression of mammalian renal transporters in Xenopus laevis oocytes.
Expression of mammalian renal transporters in Xenopus laevis oocytes.
复制标题
非洲爪蟾卵母细胞中哺乳动物肾转运蛋白的表达。
DOI：
10.1016/0003-9861(90)90622-6
复制
发表时间：
1990
影响因子：
3.9
通讯作者：
Wright,EM
中科院分区：
文献类型：
--
作者：
Coady,MJ;Pajor,AM;Toloza,EM;Wright,EM
我们将兔肾皮质的mRNA注射到异种卵母细胞中，证实了L-苯丙氨酸和l-赖氨酸的钠离子非依赖性转运载体的表达，以及l-丙氨酸和琥珀酸的钠离子依赖性转运载体的表达。肾脏氨基酸转运体的表达高峰出现在注射后6~8d。负责这四种底物运输的蛋白质是从1.5-3.0kb的mRNAs翻译而来的。这些信息是这些转运蛋白表达克隆的起点。
We have injected mRNA from rabbit renal cortex intoXenopusoocytes and demonstrated the expression of renal carriers for Na+-independent transport ofl-phenylalanine andl-lysine and Na+-dependent transport ofl-alanine and succinate. Maximal expression of renal amino acid transporters occurred 6–8 days following mRNA injection. The proteins responsible for transport of these four substrates were translated from mRNAs which are between 1.5 and 3.0 kb. This information serves as a starting point for expression cloning of these transport proteins.