Distinct Characteristics Between Perivascular and Subcutaneous Adipose-Derived Stem Cells
Distinct Characteristics Between Perivascular and Subcutaneous Adipose-Derived Stem Cells
复制标题
血管周围和皮下脂肪干细胞之间的独特特征
DOI：
10.2337/db20-1129
复制
发表时间：
2022-02-01
期刊：
影响因子：
7.7
通讯作者：
Xiang, Meixiang
中科院分区：
文献类型：
--
作者：
Xie, Yao;Ji, Yongli;Xiang, Meixiang
脂肪源性干细胞（ADSCs）可以分化为血管谱系并参与血管重塑。血管周围脂肪干细胞（PV-ADSCs）因其独特的位置而受到关注。皮下（PDSC）和腹部ADSC的异质性得到了很好的解决，但PV-ADSC的异质性尚未研究。在这项研究中，我们应用单细胞分析来比较SUB-ADSCs和PV-ADSCs的亚群、功能和细胞命运。我们发现了PV-ADSC的四个亚群（Dpp 4+、Col 4a 2 +/Icam 1+、Clec 11 a +/Cpe+和Sult 1 e 1+细胞），其中Clec 11 a+亚群可能参与并调节PV-ADSC向平滑肌细胞（SMC）表型的分化。PV-ADSCs与SUB-ADSCs具有明显的差异。
Adipose-derived stem cells (ADSCs) can differentiate into vascular lineages and participate in vascular remodeling. Perivascular ADSCs (PV-ADSCs) draw attention because of their unique location. The heterogeneity of subcutaneous (SUB) and abdominal ADSCs were well addressed, but PV-ADSCs' heterogeneity has not been investigated. In this study, we applied single-cell analysis to compare SUB-ADSCs and PV-ADSCs regarding their subpopulations, functions, and cell fates. We uncovered four subpopulations of PV-ADSCs (Dpp4+, Col4a2+/Icam1+, Clec11a+/Cpe+, and Sult1e1+ cells), among which the Clec11a+ subpopulation potentially participated in and regulated PV-ADSC differentiation toward a smooth muscle cell (SMC) phenotype. Distinct characteristics between PV-ADSCs and SUB-ADSCs were revealed.