Metabolomics of laminae and midvein during leaf senescence and source-sink metabolite management in Brassica napus L. leaves.
Metabolomics of laminae and midvein during leaf senescence and source-sink metabolite management in Brassica napus L. leaves.
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DOI：
10.1093/jxb/erx253
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发表时间：
2018-02-12
影响因子：
6.9
通讯作者：
Masclaux-Daubresse C
中科院分区：
文献类型：
--
作者：
Clément G;Moison M;Soulay F;Reisdorf-Cren M;Masclaux-Daubresse C
衰老过程中叶层和叶脉的代谢组学揭示了组织特异性和不同的衰老程序。叶子衰老是一个漫长的发育过程，对于养分管理和源库再利用非常重要。叶肉细胞的成分逐渐降解，为植物的其余部分提供营养。迄今为止，关于叶片衰老的研究还没有关注不同叶片组织的作用。在本研究中，我们从中脉解剖叶层以进行代谢物分析。叶肉细胞是营养物质的来源，在C3植物中，它们含有Rubisco作为最重要的氮储存库。静脉富含脉管系统，是所有营养物质在通过韧皮部或木质部输出之前转运、有时相互转化的地方。我们在层板和中脉中观察到的随着衰老而发生的不同代谢变化支持了这两种组织的衰老程序不同的观点。中脉中代谢物的重要积累表明，养分从源叶到库的转移主要控制在这个水平。碳和氮长距离分子，如果糖、葡萄糖、天冬氨酸和天冬酰胺，在中脉中比在纹层中更丰富。相比之下，薄片中蔗糖、谷氨酸和天冬氨酸含量更丰富。中脉中三羧酸（TCA）化合物的浓度也低于纹层中的浓度。由于氮再利用在低硝酸盐供应下增加，因此植物在两种硝酸盐浓度下生长。结果表明，除了TCA循环等一些途径外，低硝酸盐和高硝酸盐条件下与衰老相关的差异大多相似。
Metabolomics of leaf laminae and veins during ageing reveal tissue specificities and different senescence programmes. Leaf senescence is a long developmental process important for nutrient management and for source to sink remobilization. Constituents of the mesophyll cells are progressively degraded to provide nutrients to the rest of the plant. Up to now, studies on leaf senescence have not paid much attention to the role of the different leaf tissues. In the present study, we dissected leaf laminae from the midvein to perform metabolite profiling. The laminae mesophyll cells are the source of nutrients, and in C3 plants they contain Rubisco as the most important nitrogen storage pool. Veins, rich in vasculature, are the place where all the nutrients are translocated, and sometimes interconverted, before being exported through the phloem or the xylem. The different metabolic changes we observed in laminae and midvein with ageing support the idea that the senescence programme in these two tissues is different. Important accumulations of metabolites in the midvein suggest that nutrient translocations from source leaves to sinks are mainly controlled at this level. Carbon and nitrogen long-distance molecules such as fructose, glucose, aspartate, and asparagine were more abundant in the midvein than in laminae. In contrast, sucrose, glutamate, and aspartate were more abundant in laminae. The concentrations of tricarboxylic acid (TCA) compounds were also lower in the midvein than in laminae. Since nitrogen remobilization increased under low nitrate supply, plants were grown under two nitrate concentrations. The results revealed that the senescence-related differences were mostly similar under low and high nitrate conditions except for some pathways such as the TCA cycle.
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影响因子：
3.7
作者：
Lohaus, Gertrud;Schwerdtfeger, Michael
通讯作者：
Schwerdtfeger, Michael
影响因子：
5.3
作者：
Girondé A;Etienne P;Trouverie J;Bouchereau A;Le Cahérec F;Leport L;Orsel M;Niogret MF;Nesi N;Carole D;Soulay F;Masclaux-Daubresse C;Avice JC
通讯作者：
Avice JC
影响因子：
2
作者：
Holtta, T.;Mencuccini, M.;Nikinmaa, E.
通讯作者：
Nikinmaa, E.
影响因子：
7.4
作者：
Diaz, Celine;Lemaitre, Thomas;Masclaux-Daubresse, Celine
通讯作者：
Masclaux-Daubresse, Celine
影响因子：
7.2
作者：
Bohner, Anne;Kojima, Soichi;von Wiren, Nicolaus
通讯作者：
von Wiren, Nicolaus