AMELOGENIN GENE-EXPRESSION IN MOUSE INCISOR HETEROTOPIC RECOMBINATIONS
AMELOGENIN GENE-EXPRESSION IN MOUSE INCISOR HETEROTOPIC RECOMBINATIONS
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DOI：
10.1111/j.1432-0436.1989.tb00732.x
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发表时间：
1989-07-01
期刊：
影响因子：
2.9
通讯作者：
RUCH, JV
中科院分区：
文献类型：
--
作者：
AMAR, S;LUO, W;RUCH, JV
小鼠门牙器官仅在其唇表面表达负责牙釉质细胞外基质形成的基因。先前的研究表明，小鼠切牙舌内牙上皮不含潜在的成釉细胞。目前的工作扩展了我们的组织学观察，通过使用35s标记的不对称互补RNA探针从小鼠Mr特异性cDNA进行原位杂交，分析小鼠门牙组织重组中小鼠淀粉原蛋白mRNA的存在。103 amelogenin。位于舌前牙本质层前部的唇部极化成釉细胞与大量杂交信号相关，表明成釉原蛋白mRNA密度增加。相比之下，与唇前牙本质接触的舌内牙上皮未显示出淀粉原蛋白杂交信号，表明淀粉原蛋白转录物缺失。这些结果证实了我们的观察，即舌内牙上皮不含有潜在的成釉细胞，因为这些细胞即使在唇前牙提供的有利微环境中也不会转录或积累成釉原蛋白mRNA。
Mouse incisor tooth organs express the genes responsible for enamel extracellular matrix formation exclusively on the labial surface of the organ. A previous investigation has suggested that lingual inner dental epithelium of mouse incisor did not contain potential ameloblasts. The present work extends our histological observations, by analyzing the presence of mouse amelogenin mRNA in heterotopic mouse incisor tissue recombinations using in situ hybridization to 35S-labelled asymmetric complementary RNA probes from a cDNA specific to the mouse Mr 26 .times. 103 amelogenin. Labial polarized ameloblasts located in front of the lingual predentin layer were associated with numberous hybridization signals indicating an increased density of amelogenin mRNA. In contrast, the lingual inner dental epithelium in contact with labial predentin never showed amelogenin hybridization signals, indicating the absence of amelogenin transcripts. These results confirm our observation that the lingual inner dental epithelium does not contain potential ameloblasts, since these cells do not transcribe nor accumulate mRNA amelogenin even when in contract with a favorable microenvironment provided by the labial predentin.