Biofilm Formation Ability of Listeria monocytogenes Isolates from Raw Ready-to-Eat Seafood
Biofilm Formation Ability of Listeria monocytogenes Isolates from Raw Ready-to-Eat Seafood
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DOI：
10.4315/0362-028x-72.7.1476
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发表时间：
2009-07-01
影响因子：
2
通讯作者：
Kimura, Bon
中科院分区：
文献类型：
--
作者：
Takahashi, Hajime;Miya, Satoko;Kimura, Bon
单核细胞增生李斯特菌是一种食源性致病菌。许多即食食品广泛受到这种微生物的污染，并在许多国家引起了霍乱疫情和零星病例。在日本，L.单核细胞增多症污染，特别是在生的即食海鲜。伊登蒂湖单核细胞增生性李斯特氏菌亚型已经从特定商店或加工厂生产的食品样品中反复分离出来，研究人员怀疑某些李斯特氏菌。单核细胞增生分离物在这些位点形成生物膜。进行了微量滴定板生物膜形成测定，并且测试的所有生即食海鲜分离物都能够形成不同程度的生物膜。生物膜形成L. I系单核细胞增生性疾病的感染率显著高于II系单核细胞增生性疾病（P = 0.000）。然而，克隆谱系的分离物形成不同水平的生物膜，表明形成生物膜的能力受到环境因素的积极或消极影响。
Listeria monocytogenes is of great concern as a foodborne pathogen. Many ready-to-eat foods are widely contaminated with this organism and have caused listeriosis outbreaks and sporadic cases in many countries. In Japan, there is a high incidence of L. monocytogenes contamination, specifically in raw ready-to-eat seafood. Identical L. monocytogenes subtypes have been isolated repeatedly from samples of food manufactured at a given store or processing plant, and researchers suspected that certain L. monocytogenes isolates have formed biofilms at these sites. A microtiter plate biofilm formation assay was conducted, and all raw ready-to-eat seafood isolates tested were able to form biofilms to various degrees. Biofilm formation by L. monocytogenes isolates of lineage I was significantly greater (P = 0.000) than that by isolates of lineage II. However, isolates of clonal lineages formed different levels of biofilms, indicating that the ability to form a biofilm is affected positively or negatively by environmental factors.