A STUDY OF SELF-ASSEMBLY PROCESS IN A SMALL SPHERICAL VIRUS - FORMATION OF ORGANIZED STRUCTURES FROM PROTEIN SUBUNITS IN VITRO
A STUDY OF SELF-ASSEMBLY PROCESS IN A SMALL SPHERICAL VIRUS - FORMATION OF ORGANIZED STRUCTURES FROM PROTEIN SUBUNITS IN VITRO
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DOI：
10.1016/0042-6822(67)90180-8
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发表时间：
1967-01-01
期刊：
影响因子：
3.7
通讯作者：
MARKHAM, R
中科院分区：
文献类型：
--
作者：
BANCROFT, JB;HILLS, GJ;MARKHAM, R
豇豆绿斑病毒（CCMV）的干直径为250 a，其形态单位的排列与32个单位的结构一致，这些单位位于一个菱形三面体的顶点上。CCMV的水动力行为与pH值有关。粒子“膨胀”到接近中性，这样就容易受到核酸酶的攻击。经胰核糖核酸酶处理后，产生直径210 A、长度280 A的椭球状颗粒、直径160 A的二十面体小颗粒、340 A的双壳颗粒和直径160 A、端部圆角的长管。经takadi酯酶T1处理后，这些颗粒也都得到了，此外，还得到了外径为250a的双壳小球形颗粒，以及直径为250a的管，可以包覆160a的管。对这些新形式的生产进行了描述和讨论，并将产品与模型结构联系起来。此外，还概述了CCMV和溴花叶病毒可能的重组体系。
The arrangement of the morphological units of cowpea chlorotic mottle virus (CCMV), which has a dry diameter of 250 A, is consistent with that expected for a 32-unit structure, the units being situated on the vertices of a rhombic triacontahedron. The hydrodynamic behaviour of CCMV is pH dependent. The particles "swell" near neutrality and in doing so become susceptible to attack by nucleases. After treatment with pancreatic ribonuclease, ellipsoidal particles of 210 A diameter and 280 A length, small icosahedral particles of 160 A diameter, double shelled particles of 340 A, and long tubes with a diameter of 160 A and rounded ends are produced. All these particles are also found after treatment with takadiesterase T1 and in addition, small spherical particles with a double shell with an outer diameter of 250 A, and tubes with a diameter of 250 A which may enclose 160 A tubes, are produced. The production of these new forms are described and discussed and the products are related to model structures. In addition, a possible reconstitution system for CCMV and brome mosaic virus is utlined.