Identification of Type II Interferon Receptors in Geese: Gene Structure, Phylogenetic Analysis, and Expression Patterns.
Identification of Type II Interferon Receptors in Geese: Gene Structure, Phylogenetic Analysis, and Expression Patterns.
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鹅 II 型干扰素受体的鉴定：基因结构、系统发育分析和表达模式
DOI：
10.1155/2015/537637
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发表时间：
2015
影响因子：
--
通讯作者：
Cheng A
中科院分区：
文献类型：
--
作者：
Zhou H;Chen S;Qi Y;Zhou Q;Wang M;Jia R;Zhu D;Liu M;Liu F;Chen X;Cheng A
干扰素γ受体1（IFNGR1）和IFNGR2是两种属于II类细胞因子的细胞膜分子，在IFN介导的抗病毒信号通路中发挥重要作用。本文首次克隆并鉴定了鹅IFNGR1和IFNGR2。组织分布分析表明，鹅IFNγ mRNA在免疫组织中的表达量较高，主要分布在哈氏腺、盲肠扁桃体、盲肠和胸腺。IFNGR 1和IFNGR 2在鹅盲肠扁桃体中的表达水平较高，表明IFNγ在鹅的次级免疫系统中具有重要作用。在健康鹅的相同组织中，未观察到IFNγ、IFNGR1和IFNGR2表达水平之间的特异性相关性。IFNγ及其相关受体表现出不同的表达谱，尽管它们似乎保持相对平衡的状态。此外，激动剂R848导致鹅IFNγ的上调，但不影响鹅IFNGR 1或IFNGR 2的表达。总之，鹅IFNγ及其同源受体的表达趋势显示出组织特异性以及年龄相关的依赖性。这些发现可能有助于我们更好地了解鸟类对病原体的年龄相关易感性。
Interferon γ receptor 1 (IFNGR1) and IFNGR2 are two cell membrane molecules belonging to class II cytokines, which play important roles in the IFN-mediated antiviral signaling pathway. Here, goose IFNGR1 and IFNGR2 were cloned and identified for the first time. Tissue distribution analysis revealed that relatively high levels of goose IFNγ mRNA transcripts were detected in immune tissues, including the harderian gland, cecal tonsil, cecum, and thymus. Relatively high expression levels of both IFNGR1 and IFNGR2 were detected in the cecal tonsil, which implicated an important role of IFNγ in the secondary immune system of geese. No specific correlation between IFNγ, IFNGR1, and IFNGR2 expression levels was observed in the same tissues of healthy geese. IFNγ and its cognate receptors showed different expression profiles, although they appeared to maintain a relatively balanced state. Furthermore, the agonist R848 led to the upregulation of goose IFNγ but did not affect the expression of goose IFNGR1 or IFNGR2. In summary, trends in expression of goose IFNγ and its cognate receptors showed tissue specificity, as well as an age-related dependency. These findings may help us to better understand the age-related susceptibility to pathogens in birds.