A genome-wide survey of HD-Zip genes in rice and analysis of drought-responsive family members
A genome-wide survey of HD-Zip genes in rice and analysis of drought-responsive family members
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DOI：
10.1007/s11103-007-9255-7
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发表时间：
2008-01-01
影响因子：
5.1
通讯作者：
Ouwerkerk, Pieter B. F.
中科院分区：
文献类型：
--
作者：
Agalou, Adamantia;Purwantomo, Sigit;Ouwerkerk, Pieter B. F.
同源结构域亮氨酸拉链（HD-Zip）基因编码的转录因子在植物发育中具有多种功能，通常与逆境适应有关。HD-Zip基因是植物中最丰富的同源盒（HB）基因，不存在于其他真核生物中。本文报道了水稻HD-Zip家族I、II和III的完整注释，并与拟南芥进行了系统发育重建。在保守的内含子-外显子结构支持下，基于邻域连接和最大简约（MP）树预测水稻和拟南芥HD-Zip基因的同源对。此外，许多HD-Zip基因似乎是水稻独有的。对EST和cDNA数据库的检索和RT-PCR的表达分析表明，31个水稻HD-Zip预测基因中有30个得到了表达。大多数HD-Zip基因在成熟植物和幼苗中广泛表达，但其他基因表现出更多的器官特异性模式。与拟南芥和其他薯类植物一样，水稻HD-Zip I和II基因的一个子集被发现受到干旱胁迫的调节。我们鉴定了干旱诱导和干旱抑制的HD-Zip基因，并证明这些基因在干旱敏感型和耐旱型水稻品种中受到不同的调控。选择干旱抑制HD-Zip家族I基因Oshox4进行启动子gus分析，结果表明Oshox4的干旱响应性受启动子控制，并且Oshox4的表达主要是血管特异性的。Oshox4的功能缺失分析没有显示出特定的表型，但过表达分析表明Oshox4在伸长和成熟过程中起作用。
The homeodomain leucine zipper (HD-Zip) genes encode transcription factors that have diverse functions in plant development and have often been implicated in stress adaptation. The HD-Zip genes are the most abundant group of homeobox (HB) genes in plants and do not occur in other eukaryotes. This paper describes the complete annotation of the HD-Zip families I, II and III from rice and compares these gene families with Arabidopsis in a phylogeny reconstruction. Orthologous pairs of rice and Arabidopsis HD-Zip genes were predicted based on neighbour joining and maximum parsimony (MP) trees with support of conserved intron-exon organization. Additionally, a number of HD-Zip genes appeared to be unique to rice. Searching of EST and cDNA databases and expression analysis using RT-PCR showed that 30 out of 31 predicted rice HD-Zip genes are expressed. Most HD-Zip genes were broadly expressed in mature plants and seedlings, but others showed more organ specific patterns. Like in Arabidopsis and other dicots, a subset of the rice HD-Zip I and II genes was found to be regulated by drought stress. We identified both drought-induced and drought-repressed HD-Zip genes and demonstrate that these genes are differentially regulated in drought-sensitive versus drought-tolerant rice cultivars. The drought-repressed HD-Zip family I gene, Oshox4, was selected for promoter-GUS analysis, showing that drought-responsiveness of Oshox4 is controlled by the promoter and that Oshox4 expression is predominantly vascular-specific. Loss-of-function analysis of Oshox4 revealed no specific phenotype, but overexpression analysis suggested a role for Oshox4 in elongation and maturation processes.