Intensity correlation analysis of Raman spectra of concentrated Ficoll solutions
Intensity correlation analysis of Raman spectra of concentrated Ficoll solutions
复制标题
浓缩聚蔗糖溶液拉曼光谱的强度相关分析
DOI：
10.1117/12.2609947
复制
发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Boukari, Hacene
中科院分区：
文献类型：
--
作者：
Rivera-Lopez, Yahira M.;Salih, Mohamed A.;Boukari, Fatima;Barnett, Cleon M.;Boukari, Hacene
细胞内环境中挤满了各种生物大分子(~80-400 mg/ml)，可能影响蛋白质折叠、小分子结合、酶活性和病理性蛋白质聚集等各种生物学过程。作为一个模型，我们一直在使用Ficoll的溶液来模拟环境，Ficoll是一种高度分枝的多糖。除了生物医学应用(如血液分离)外，它还被用作大分子Crowder，用于蛋白质折叠和稳定性、细胞体积信号、组织工程和纳米传输的研究。在这项研究中，我们的目标是识别和评估与Ficoll分子和Ficoll-Ficoll相互作用相关的拉曼光谱特征，以用于未来拥挤效应的研究。除了水(~1640 cm~(-1)和~3200 cm~(-1))、溶解O_2(~1556 cm~(-1))和N_2(~2331 cm~(-1))的拉曼峰外，我们还在1500-3500 cm~(-1)波数范围内发现了一个明显的拉曼峰(~2900 cm~(-1))，它与Ficoll、CH和CH_2伸缩模式有关。随着Ficoll浓度的增加，Ficoll拉曼峰的强度增加，而水拉曼峰的强度减小，后者可能是由于水含量的减少所致。此外，我们应用强度相关分析(ICA)方法评估了拉曼光谱随Ficoll浓度(高达1000 mg/ml)的系统变化。ICA显示了整个波数范围内的整体线性趋势，但也显示了闭合环路，这可以归因于某些峰的轮廓的轻微变化。结果表明，在拥挤的生物样品的化学分析中，ICA是一种潜在的有洞察力的工具来识别Ficoll。
The intracellular environment is crowded with diverse biomacrolecules (~80-400 mg/ml), likely affecting various biological processes such as protein folding, binding of small molecules, enzymatic activity, and pathological protein aggregation. As a model we have been using solutions of Ficoll, a highly branched polysaccharide, to mimic the environment. Besides its biomedical applications (e.g. blood separation), it has been used as a macromolecular crowder in studies of protein folding and stability, cell volume signaling, tissue engineering, and nanotransport. In this study, our goal is to identify and assess Raman spectral signatures associated with Ficoll molecules and Ficoll-Ficoll interactions for future investigations of crowding effects. In addition to the Raman peaks of water (~1640 cm-1and ~3200 cm-1) and dissolved O2(~1556 cm-1) and N2 (~2331 cm-1) we identified a distinct Raman peak (~2900 cm-1) in the 1500-3500 cm-1wavenumber range, which is associated with Ficoll and CH and CH2stretching modes. As the Ficoll concentration increases, the intensity of the Ficoll Raman peaks increases while the intensity of the water Raman peaks decreases, the latter likely due to reduction of water content. Further, we have applied the intensity correlation analysis (ICA) method to assess systematic changes of Raman spectra with Ficoll concentration (up to 1000 mg/ml). ICA indicates an overall linear trend over the full wavenumber range, but also shows closed loops that can be attributed to slight changes of the profiles of certain peaks. The results demonstrate ICA as a potential insightful tool for identifying Ficoll in chemical analysis of crowded biological samples.
DOI：
10.1152/ajprenal.00097.2007
发表时间：
2007-10-01
影响因子：
4.2
作者：
Fissell, William H.;Manley, Sargum;Roy, Shuvo
通讯作者：
Roy, Shuvo
影响因子：
8
作者：
Tokuriki, N;Kinjo, M;Yomo, T
通讯作者：
Yomo, T