Determination of honokiol and magnolol by micro HPLC with electrochemical detection and its application to the distribution analysis in branches and leaves of Magnolia obovata.
Determination of honokiol and magnolol by micro HPLC with electrochemical detection and its application to the distribution analysis in branches and leaves of Magnolia obovata.
复制标题
DOI：
10.1248/cpb.53.319
复制
发表时间：
2005-03
影响因子：
1.7
通讯作者：
Akira Kotani;S. Kojima;H. Hakamata;D. Jin;F. Kusu
中科院分区：
文献类型：
--
作者：
Akira Kotani;S. Kojima;H. Hakamata;D. Jin;F. Kusu
建立了测定鲜厚朴中和厚朴酚和厚朴酚含量的方法。通过微型高效液相色谱电化学检测器（microHPLC-ECD）测定。采用Capcell Pak C-18 UG 120微孔十八烷基硅胶（ODS）柱，甲醇-水-磷酸（65：35：0.5，v/v/v）作为移动的流动相，在+0.8 V vs. Ag/AgCl下施加电位进行色谱分析。峰高与和厚朴酚和厚朴酚的进样量在0.67 ~ 2.0ng范围内呈线性关系（r>0.999）。检出限（S/N=3）为0.13pg。测定和厚朴酚和厚朴酚的相对标准偏差分别为0.73%和1.17%（n=5）。采用70%甲醇提取厚朴药材中的和厚朴酚和厚朴酚，用移动的相稀释，进样至microHPLC-ECD中进行测定。厚朴中厚朴酚和厚朴酚的回收率均大于98.7%，RSD均小于0.93%（n=5）。测定了和厚朴酚和厚朴酚在鲜厚朴树皮、韧皮部、木材、叶片和叶柄中的分布。obovata使用40-238 mg的重量样品制备。该方法可用于厚朴中和厚朴酚和厚朴酚的含量测定。obovata，它是日本传统草药的天然黄柏的候选者。
A simple and sensitive method has been developed for determining honokiol and magnolol in fresh Magnolia obovata (M. obovata) by micro high-performance liquid chromatography with electrochemical detection (microHPLC-ECD). Chromatography was performed using a Capcell Pak C-18 UG 120 microbore octadecylsilica (ODS) column, methanol-water-phosphoric acid (65 : 35 : 0.5, v/v/v), as a mobile phase and applied potential at +0.8 V vs. Ag/AgCl. Peak heights were found linearly related to the amounts of honokiol and magnolol injected from 0.67 pg to 2.0 ng (r>0.999). The detection limits (S/N=3) were 0.13 pg, respectively. Honokiol and magnolol of 0.27 ng were detected with relative standard deviation (RSD) of 0.73 and 1.17% (n=5), respectively. Honokiol and magnolol in Magnolia Bark of the Japanese Pharmacopoeia were extracted with 70% methanol, diluted with a mobile phase, and injected into the microHPLC-ECD for determination. Recoveries of honokiol and magnolol in Magnolia Bark exceeded 98.7% with RSD, less than 0.93% (n=5). Determination of the distributions of honokiol and magnolol in bark, phloem, wood, leaf blades, and petioles of fresh M. obovata were made using weight samples of 40-238 mg. This method is useful to determine honokiol and magnolol in M. obovata, which is a candidate for crude magnolia bark for traditional Japanese herbal medicines.