Sequence characterization and expression patterns of two defensin-like antimicrobial peptides from the tick Haemaphysalis longicornis
Sequence characterization and expression patterns of two defensin-like antimicrobial peptides from the tick Haemaphysalis longicornis
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DOI：
10.1016/j.peptides.2007.04.019
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发表时间：
2007-06-01
期刊：
影响因子：
3
通讯作者：
Fujisaki, Kozo
中科院分区：
文献类型：
--
作者：
Zhou, Jinlin;Liao, Min;Fujisaki, Kozo
从长角血蜱（Haemaphysalis longicornis）中克隆了两个编码防御素样抗菌肽的cDNA序列。Higut-defensin（H. Lorigicornis midgut defensin）全长333 bp，编码73个氨基酸。Hisal-defensin（H. longicornis salivary gland defensin）全长382 bp，编码81个氨基酸。基于Hlgut-防御素和Hlsal-防御素序列的合成肽对多种革兰氏阳性和革兰氏阴性细菌进行了抗菌活性测试。利用实时荧光定量PCR技术检测了两种防御素样肽的组织特异性表达，并发现脂多糖（LPS）注射能显著诱导Hlgut-defensin和Hlsal-defensin基因转录。(c)2007年爱思唯尔公司All rights reserved.
Two cDNAs encoding defensin-like antimicrobial peptides were cloned and sequenced from the tick Haemaphysalis longicornis. The full-length cDNA of Higut-defensin (H. lorigicornis midgut defensin) is 333 bp, encoding an expected protein with 73 amino acids. The full-length cDNA of Hisal-defensin (H. longicornis salivary gland defensin) is 382 bp, encoding an expected protein with 81 amino acids. The antibacterial activities of the synthetic peptides based on the Hlgut-defensin and Hlsal-defensin sequences were tested against a variety of Gram-positive and Gram-negative bacteria. Using real-time PCR, the tissue-specific expression of two defensin-like peptides were determined and it was also found that the gene transcripts of Hlgut-defensin and Hlsal-defensin were significantly induced by a lipopolysaccharide (LPS) injection. (c) 2007 Elsevier Inc. All rights reserved.