Adeno-associated virus vectors preferentially transduce cells in S phase.
Adeno-associated virus vectors preferentially transduce cells in S phase.
复制标题
腺相关病毒载体优先转导S期细胞。
DOI：
10.1073/pnas.91.19.8915
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发表时间：
1994
影响因子：
11.1
通讯作者：
Alexander,IE
中科院分区：
文献类型：
--
作者：
Russell,DW;Miller,AD;Alexander,IE
基于腺相关病毒的载体可以通过染色体整合在受体细胞中稳定地转移基因。在这项研究中，我们感染了静止和分裂的原代人成纤维细胞培养与腺相关病毒载体编码碱性磷酸酶和新霉素磷酸转移酶。我们发现S期细胞的转导频率约为非S期细胞的200倍。然而，S期和有丝分裂都不是转导所必需的。单链载体基因组在静止培养物中存活，并且可以通过刺激这些培养物分裂来募集用于转导。稳定的转导子含有随机整合的载体序列。这些发现对腺相关病毒载体在基因治疗中的应用具有重要意义。
Vectors based on adeno-associated virus can stably transfer genes by chromosomal integration in recipient cells. In this study we have infected stationary and dividing primary human fibroblast cultures with adeno-associated virus vectors encoding alkaline phosphatase and neomycin phosphotransferase. We find that the transduction frequency of S phase cells is about 200 times that of non-S phase cells. However, neither S phase nor mitosis is essential for transduction. Single-stranded vector genomes survive in stationary cultures and can be recruited for transduction by stimulating these cultures to divide. Stable transductants contain randomly integrated vector sequences. These findings have important implications for the use of adeno-associated virus vectors in gene therapy.