Laser and tandem scanning confocal microscopic studies of rabbit corneal wound healing.
Laser and tandem scanning confocal microscopic studies of rabbit corneal wound healing.
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DOI：
10.1002/sca.4950160304
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
H. Ichijima;J. V. Jester;W. Petroll;H. D. Cavanagh
中科院分区：
文献类型：
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作者：
H. Ichijima;J. V. Jester;W. Petroll;H. D. Cavanagh
应用激光共聚焦显微镜（LSCM）和串联扫描共聚焦显微镜（TSCM）观察角膜内皮损伤后的愈合过程。采用离体激光扫描共聚焦显微镜和在体激光扫描共聚焦显微镜观察兔角膜冷冻损伤后的变化。激光共聚焦显微镜显示了细胞内肌动蛋白丝的组织，鬼笔环肽-FITC染色，在迁移的内皮细胞，转化成纤维细胞样细胞，基质角膜细胞，上皮细胞在角膜组织的伤口愈合过程中。TSCM提供了在同一角膜上无创性伤口愈合的体内细胞修复过程中从角膜内皮到上皮的形态学变化的连续空间观察，而无需动物处死。离体LSCM支持体内TSCM观察结果的形态学分析。
The process of corneal endothelial wound healing was studied using laser and tandem scanning confocal microscopy (LSCM and TSCM). Following transcorneal freeze (TCF) injury, rabbit corneas were observed using ex vivo LSCM and in vivo TSCM. LSCM revealed the intracellular actin filament organization which, stained with phalloidin-FITC, in migrating endothelial cells, transformed fibroblast-like cells, stroma keratocytes, and epithelial cells during wound healing in corneal tissue. The TSCM provided sequential spatial observation of morphologic changes from endothelium to epithelium of the cornea during in vivo cellular repair of wound healing noninvasively on the same cornea without animal sacrifice. Ex vivo LSCM supported the morphologic analysis of the in vivo TSCM observations.