Substrate perturbation alters the glycoside hydrolase activities and community composition of switchgrass-adapted bacterial consortia
Substrate perturbation alters the glycoside hydrolase activities and community composition of switchgrass-adapted bacterial consortia
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DOI：
10.1002/bit.24388
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发表时间：
2012-05-01
影响因子：
3.8
通讯作者：
Singer, Steven W.
中科院分区：
文献类型：
--
作者：
Gladden, John M.;Eichorst, Stephanie A.;Singer, Steven W.
细菌响应木质纤维素生物质组成的变化而调节糖苷水解酶的表达。柳枝稷适应的嗜热细菌聚生体的扰动与各种生物质底物的响应进行了表征，以确定是否细菌聚生体也响应生物质组成的变化。柳枝稷适应财团与这些替代底物的孵育产生的糖苷水解酶活性和细菌群落组成的变化。在与微晶纤维素和三氟乙酸预处理的柳枝稷一起孵育后，观察到内切葡聚糖酶活性显著增加。与此相反，培养的微生物聚生体与离子液体预处理柳枝稷木聚糖酶活性显着增加。这些培养物的微生物群落分析表明，增加的内切葡聚糖酶活性与海洋红嗜热菌相关的细菌的增加相关。在培养基中加入简单的有机底物，可消除糖苷水解酶活性，并富集与嗜热栖热菌相关的细菌。这些结果表明，生物质底物的组成影响糖苷水解酶活性和生物质解构细菌聚生体的群落组成。Biotechnol. Bioeng. 2012; 109：11401145。(C)2011 Wiley Periodicals，Inc.
Bacteria modulate glycoside hydrolase expression in response to the changes in the composition of lignocellulosic biomass. The response of switchgrass-adapted thermophilic bacterial consortia to perturbation with a variety of biomass substrates was characterized to determine if bacterial consortia also responded to changes in biomass composition. Incubation of the switchgrass-adapted consortia with these alternative substrates produced shifts in glycoside hydrolase activities and bacterial community composition. Substantially increased endoglucanase activity was observed upon incubation with microcrystalline cellulose and trifluororacetic acid-pretreated switchgrass. In contrast, culturing the microbial consortia with ionic liquid-pretreated switchgrass increased xylanase activity dramatically. Microbial community analyses of these cultures indicated that the increased endoglucanase activity correlated with an increase in bacteria related to Rhodothermus marinus. Inclusion of simple organic substrates in the culture medium abrogated glycoside hydrolase activity and enriched for bacteria related to Thermus thermophilus. These results demonstrate that the composition of biomass substrates influences the glycoside hydrolase activities and community composition of biomass-deconstructing bacterial consortia. Biotechnol. Bioeng. 2012; 109:11401145. (C) 2011 Wiley Periodicals, Inc.