Laponite Nanodisks as an Efficient Platform for Doxorubicin Delivery to Cancer Cells
Laponite Nanodisks as an Efficient Platform for Doxorubicin Delivery to Cancer Cells
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Laponite Nanodisks 作为阿霉素向癌细胞输送的有效平台
DOI：
10.1021/la4001363
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发表时间：
2013-04-23
期刊：
影响因子：
3.9
通讯作者：
Shi, Xiangyang
中科院分区：
文献类型：
--
作者：
Wang, Shige;Wu, Yilun;Shi, Xiangyang
我们报告了一种使用合成锂皂石（Laponite）纳米盘作为平台，将多柔比星（DOX）有效递送至癌细胞的简便方法。在这项研究中，DOX通过离子交换过程被封装到石墨烯的层间空间中，具有98.3 +/-0.77%的极高负载效率。成功的DOX负载通过不同的方法进行了广泛的表征。体外药物释放研究表明，DOX从纳米片中的释放具有pH依赖性，在酸性pH（pH = 5.4）条件下释放速度比生理pH条件下快。重要的是，细胞活力测定结果显示，在相同的DOX浓度下，与游离DOX药物相比，NH3/DOX纳米盘在抑制模型癌细胞系（人上皮癌细胞，KB细胞）的生长方面显示出高得多的治疗功效。增强的抗肿瘤功效主要是由于与游离D 0X相比，NH 4/D 0X纳米盘的细胞摄取多得多，这已经通过共聚焦激光扫描显微镜和流式细胞术分析证实。高DOX有效载荷和增强的抗肿瘤功效使纳米盘成为用于不同生物医学应用的稳健载体系统。
We report a facile approach to using laponite (LAP) nanodisks as a platform for efficient delivery of doxorubicin (DOX) to cancer cells. In this study, DOX was encapsulated into the interlayer space of LAP through an ionic exchange process with an exceptionally high loading efficiency of 98.3 +/- 0.77%. The successful DOX loading was extensively characterized via different methods. In vitro drug release study shows that the release of DOX from LAP/DOX nanodisks is pH-dependent, and DOX is released at a quicker rate at acidic pH condition (pH = 5.4) than at physiological pH condition. Importantly, cell viability assay results reveal that LAP/DOX nanodisks display a much higher therapeutic efficacy in inhibiting the growth of a model cancer cell line (human epithelial carcinoma cells, KB cells) than free DOX drug at the same DOX concentration. The enhanced antitumor efficacy is primarily due to the much more cellular uptake of the LAP/DOX nanodisks than that of free DOX, which has been confirmed by confocal laser scanning microscope and flow cytometry analysis. The high DOX payload and enhanced antitumor efficacy render LAP nanodisks as a robust carrier system for different biomedical applications.