The Role of Polymer Nanolayer Architecture on the Separation Performance of Anion-Exchange Membrane Adsorbers: Part II. DNA and Virus Separations
The Role of Polymer Nanolayer Architecture on the Separation Performance of Anion-Exchange Membrane Adsorbers: Part II. DNA and Virus Separations
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DOI：
10.1002/bit.23222
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发表时间：
2011-11-01
影响因子：
3.8
通讯作者：
Husson, Scott M.
中科院分区：
文献类型：
--
作者：
Bhut, Bharat V.;Weaver, Justin;Husson, Scott M.
第一部分中开发的表面引发聚合方案用于制备具有可变聚合物链接枝密度和聚合度的强阴离子交换膜，用于DNA和病毒颗粒分离。第二部分的重点是评估聚合物纳米层结构在DNA和病毒结合中的作用。以鲑鱼精子dna （SS-DNA）为模型核酸，测定10%突破时的动态结合能力。动态结合能力随着聚（[2-（甲基丙烯氧基）乙基]三甲基氯化铵）链密度的增加而线性增加，直至本研究中使用的最高链密度。新膜的ss - dna结合能力（30 mg/mL）比具有相同官能团化学的领先商用膜高三倍。结合DNA的洗脱产生一个尖峰，导致相对于饲料浓度增加13倍。这种浓度效应进一步证明了新设计的q型膜具有良好的传输性能。然而，与第一部分关于蛋白质结合的发现不同，SS-DNA结合不是完全可逆的。以小鼠微小病毒（MVM）为模型病毒，对新设计的q型膜的病毒清除性能进行了评价。随着聚合物链密度的增加，MVM的病毒对数降低率（LRV）增加。对于给定的MVM杂质负载，膜表现出bbb4.5 LRV，并且可能具有更高的LRV值，因为这些样品的流动分数中的MVM浓度低于该试验的检测极限。Biotechnol。Bioeng。2011;108: 2654 - 2660。(C) 2011 Wiley期刊公司
The surface-initiated polymerization protocol developed in part I was used to prepare strong anion-exchange membranes with variable polymer chain graft densities and degrees of polymerization for DNA and virus particle separations. A focus of part II was to evaluate the role of polymer nanolayer architecture on DNA and virus binding. Salmon sperm-DNA (SS-DNA) was used as model nucleic acid to measure the dynamic-binding capacities at 10% breakthrough. The dynamic-binding capacity increases linearly with increasing poly ([2-(methacryloyloxy)ethyl]trimethylammonium chloride) chain density up to the highest chain density used in this study. The new membranes yielded threefold higher SS-DNA-binding capacity (30 mg/mL) than a leading commercial membrane with the same functional group chemistry. Elution of bound DNA yielded a sharp peak, and resulted in a 13-fold increase relative to the feed concentration. This concentration effect further demonstrates the highly favorable transport properties of the newly designed Q-type membranes. However, unlike findings in part I on protein binding, SS-DNA binding was not fully reversible. Minute virus of mice (MVM) was used as model virus to evaluate the virus clearance performance of newly designed Q-type membranes. Log reduction of virus (LRV) of MVM increased with increasing polymer chain density. Membranes exhibited >4.5 LRV for the given MVM impurity load and may be capable of higher LRV values, as the MVM concentration in the flow-through fraction of these samples was below the limit of detection of the assay. Biotechnol. Bioeng. 2011; 108: 2654-2660. (C) 2011 Wiley Periodicals, Inc.