Microsyringe-based deposition of two-dimensional and three-dimensional polymer scaffolds with a well-defined geometry for application to tissue engineering
Microsyringe-based deposition of two-dimensional and three-dimensional polymer scaffolds with a well-defined geometry for application to tissue engineering
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DOI：
10.1089/107632702320934182
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发表时间：
2002-12-01
影响因子：
--
通讯作者：
Ahluwalia, A
中科院分区：
文献类型：
--
作者：
Vozzi, G;Previti, A;Ahluwalia, A
描述了一种在特定和复杂的结构中控制生物材料和细胞沉积的技术。它使用高精度的三维微定位系统和压力控制注射器来沉积横向分辨率为5微米的生物聚合物结构。压力激活的微型注射器配备了一个细孔出口针和各种各样的二维和三维图案，待沉积的细胞可以附着在这些图案上。通过施加压力、电机转速、线宽和高度以及聚合物粘度等沉积参数对系统进行了表征，并建立了模拟沉积过程的流体动力学模型，从而能够准确地预测聚合物结构的拓扑特征。
A technique for controlled deposition of biomaterials and cells in specific and complex architectures is described. It employs a highly accurate three-dimensional micropositioning system with a pressure-controlled syringe to deposit biopolymer structures with a lateral resolution of 5 mum. The pressure-activated microsyringe is equipped with a fine-bore exit needle and a wide variety of two- and three-dimensional patterns on which cells to be deposited can adhere. The system has been characterized in terms of deposition parameters such as applied pressure, motor speed, line width and height, and polymer viscosity, and a fluid dynamic model simulating the deposition process has been developed, allowing an accurate prediction of the topological characteristics of the polymer structures.