A method to measure nanomechanical properties of biological objects
A method to measure nanomechanical properties of biological objects
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DOI：
10.1063/1.4858411
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发表时间：
2013-12-23
影响因子：
4
通讯作者：
Szoszkiewicz, Robert
中科院分区：
文献类型：
--
作者：
Ploscariu, Nicoleta;Szoszkiewicz, Robert
我们假设，一个将能够定量地推断蛋白质，细胞和其他生物体（BO）的机械性能的变化，通过测量与BO接触的原子力显微镜杠杆的几个热激发共振频率的变化。在这里，我们提供了一种方法来提取生物相关的磷酸盐缓冲盐水介质中的BO的弹簧常数和分子阻尼因子，并使用具有小的纵横比（长度与宽度的比率）的顺应性AFM杠杆。(C)2013 AIP Publishing LLC.
We postulate that one will be able to quantitatively infer changes in the mechanical properties of proteins, cells, and other biological objects (BO) by measuring the shifts of several thermally excited resonance frequencies of atomic force microscopy cantilevers in contact with BOs. Here, we provide a method to extract spring constants and molecular damping factors of BOs in biologically relevant phosphate buffered saline medium and using compliant AFM cantilevers with a small aspect ratio (a ratio of length to width). (C) 2013 AIP Publishing LLC.