Prevalence of low-level hepatitis B viremia in patients with HBV surface antigen-negative hepatocellular carcinoma with and without hepatitis C virus infection in Japan: Analysis by COBAS TaqMan real-time PCR
Prevalence of low-level hepatitis B viremia in patients with HBV surface antigen-negative hepatocellular carcinoma with and without hepatitis C virus infection in Japan: Analysis by COBAS TaqMan real-time PCR
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DOI：
10.1159/000101911
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发表时间：
2007-01-01
期刊：
影响因子：
4.6
通讯作者：
Kanamori, Akira
中科院分区：
文献类型：
--
作者：
Toyoda, Hidenori;Kumada, Takashi;Kanamori, Akira
目的：循环低水平B肝炎病毒（HBV），定义为“隐匿性HBV感染”的状态之一，对HBV表面抗原（HBsAg）阴性患者肝细胞癌（HCC）的发展的影响存在争议。此外，隐匿性HBV感染的流行在很大程度上取决于HBV检测方法的灵敏度。我们使用一种新开发的基于实时聚合酶链反应的敏感方法，调查了HBsAg阴性的HCC患者血清中低水平HBV的患病率。研究方法：采用COBAS TaqMan HBV检测试剂盒对132例HBsAg阴性HCC患者（95例丙型肝炎病毒[ HCV]感染，37例未检测到肝炎病毒感染）的血清进行了HBV DNA检测，其中95%的命中率为35拷贝/ml（6.7 IU/ ml）。结果：95例HCV相关性肝癌中2例（2.1%）检出低水平HBV DNA，37例非病毒性肝癌中1例（2.7%）检出低水平HBV DNA。结论：即使使用最敏感的HBV检测方法，在HBsAg阴性的HCV感染的HCC患者和未检测到肝炎病毒的患者中，检测到循环低水平HBV的患病率也很低。循环中低水平的HBV在HBsAg阴性的HCC中的肝癌发生中似乎没有发挥重要作用。版权所有（C）2007 S. Karger AG，巴塞尔。
Objectives: The effect of circulating low-level hepatitis B virus ( HBV), defined as one of the states of 'occult HBV infection', on the development of hepatocellular carcinoma ( HCC) in HBV surface antigen (HBsAg)-negative patients is controversial. In addition, the prevalence of occult HBV infection strongly depends on the sensitivity of the HBV detection method. We investigated the prevalence of low-level HBV in the serum of HBsAg-negative patients with HCC using a newly developed, sensitive method based on real-time polymerase chain reaction. Methods: Serum was examined for HBV DNA in 132 patients with HBsAg-negative HCC ( 95 with hepatitis C virus [ HCV] infection and 37 without detectable hepatitis virus infection) with the COBAS TaqMan HBV test, of which the 95% hit rate is 35 copies/ml ( 6.7 IU/ ml). Results: Low-level HBV DNA was detected in 2 of 95 (2.1%) patients with HCV-related HCC and 1 of 37 (2.7%) patients with nonviral HCC. Conclusion: The prevalence of the detection of circulating low-level HBV was low in both HBsAg-negative HCC patients with HCV infection and those without detectable hepatitis virus, even with the use of the most sensitive method for the detection of HBV. Circulating low-level HBV does not appear to play an important role in hepatocarcinogenesis in HBsAg-negative HCC. Copyright (C) 2007 S. Karger AG, Basel.