A novel food-grade lactococcal expression system and its use for secretion and delivery of an oral vaccine antigen
A novel food-grade lactococcal expression system and its use for secretion and delivery of an oral vaccine antigen
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一种新型食品级乳球菌表达系统及其用于分泌和递送口服疫苗抗原的用途
DOI：
10.1002/jctb.5536
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发表时间：
2018
影响因子：
3.4
通讯作者：
Qingtang Fan
中科院分区：
文献类型：
--
作者：
Rongguang Zhang;Dan Qiao;Xiaoyan Peng;Guangcai Duan;Qingfeng Shi;Linghan Zhang;Chen Wang;Wenjuan Liang;Shuaiyin Chen;Qingtang Fan
食品级细菌表达系统相对较少，越来越多的证据表明，细菌载体疫苗中抗原的亚细胞定位可能会显著影响免疫效力。本研究开发了一种用于异源蛋白生产和口服疫苗递送的新型食品级分泌表达系统。利用该表达系统，获得了一株L.分泌乳杆菌H.构建pyloriUreB，并用于接种SPF BALB/c小鼠。结果表明，UreB在L.在工程菌株的细胞裂解物和培养上清液中均检测到乳酸杆菌，约占培养上清液蛋白的50%，并被小鼠抗H.幽门裂用工程L.乳酸菌可显著提高小鼠抗UreB血清抗体水平（P< 0.05）。乳酸分泌表达系统，这可能对食用和药用蛋白生产以及口服疫苗开发具有明显的潜在影响。此外，这是第一次报告分泌表达的aH。pylori抗原，并且分泌UreB的工程菌株可以是一种可分泌的H。pylori疫苗候选者。© 2017化学工业协会
BACKGROUNDFood‐grade bacterial expression systems are relatively rare, and increasing evidence indicates that subcellular location of antigens in bacterial vector vaccines may markedly affect the immune efficacy.RESULTSThis study developed a novel food‐grade secretory expression system for heterologous protein production and oral vaccine delivery. Furthermore, by using the expression system, an engineeredL. lactisstrain secretingH. pyloriUreB was constructed, and used to vaccinate SPF BALB/c mice. As results, the UreB expressed inL. lactiswas detected in both cell lysates and culture supernatant of the engineered strain, constituting roughly 50% of the culture supernatant proteins, and recognized by mouse anti‐H. pylorisera. Oral vaccination with the engineeredL. lactisproduced a significantly elevated anti‐UreB serum antibody level in mice (P< 0.05).CONCLUSIONThese data show a novel food‐gradeL. lactissecretory expression system, which may have distinct potential impact on edible and medicinal protein production and oral vaccine development. Moreover, this is the first report on secretory expression of aH. pyloriantigen via using a food‐grade lactococcal expression system, and the engineered strain secreting UreB can be a hopefulH. pylorivaccine candidate. © 2017 Society of Chemical Industry