Infusion of mature megakaryocytes into mice yields functional platelets
Infusion of mature megakaryocytes into mice yields functional platelets
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DOI：
10.1172/jci43326
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发表时间：
2010-11-01
影响因子：
15.9
通讯作者：
Poncz, Mortimer
中科院分区：
文献类型：
--
作者：
Fuentes, Rudy;Wang, Yuhuan;Poncz, Mortimer
血小板生成是巨核细胞产生循环血小板的过程，目前尚不完全清楚。先前的研究表明巨核细胞在肺血管系统中脱落血小板。为了更好地了解血小板生成并开发一种不依赖供体（供体仍然短缺）的潜在血小板输注策略，我们检查了输注到小鼠体内的巨核细胞是否会脱落血小板。输注的巨核细胞导致临床相关的血小板数量增加。释放的血小板大小正常，显示适当的表面标记，并且具有接近正常的循环半衰期。供体来源的血小板的功能也在体内得到证实。输注的巨核细胞主要定位于肺血管系统，在那里它们似乎会脱落血小板。这些数据表明，可能不需要从离体生长的巨核细胞产生血小板来实现临床相关的血小板数量增加。
Thrombopoiesis, the process by which circulating platelets arise from megakaryocytes, remains incompletely understood. Prior studies suggest that megakaryocytes shed platelets in the pulmonary vasculature. To better understand thrombopoiesis and to develop a potential platelet transfusion strategy that is not dependent upon donors, of which there remains a shortage, we examined whether megakaryocytes infused into mice shed platelets. Infused megakaryocytes led to clinically relevant increases in platelet numbers. The released platelets were normal in size, displayed appropriate surface markers, and had a near-normal circulating half-life. The functionality of the donor-derived platelets was also demonstrated in vivo. The infused megakaryocytes mostly localized to the pulmonary vasculature, where they appeared to shed platelets. These data suggest that it may be unnecessary to generate platelets from ex vivo grown megakaryocytes to achieve clinically relevant increases in platelet numbers.