Twelve years' detection of respiratory viruses by immunofluorescence in hospitalised children: impact of the introduction of a new respiratory picornavirus assay
Twelve years' detection of respiratory viruses by immunofluorescence in hospitalised children: impact of the introduction of a new respiratory picornavirus assay
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DOI：
10.1186/1471-2334-11-41
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发表时间：
2011-02-07
影响因子：
3.7
通讯作者：
Barbani, Maria Teresa
中科院分区：
文献类型：
--
作者：
Sadeghi, Christine D.;Aebi, Christoph;Barbani, Maria Teresa
背景资料：直接免疫荧光法（DFA）是一种快速、廉价的呼吸道病毒检测方法，因此可用于监测。仅基于DFA发表的流行病学研究很少，并且没有包括呼吸道小核糖核酸病毒和人偏肺病毒（hMPV）。我们希望评估使用DFA的流行病学研究与长期观察呼吸道病毒，包括呼吸道小核糖核酸病毒和hMPV。方法：自1998年以来，所有儿童呼吸道疾病住院伯尔尼大学医院已与DFA筛选常见的呼吸道病毒，包括腺病毒，呼吸道合胞病毒（RSV），流感A和B，副流感病毒1-3。2006年，增加了呼吸道小核糖核酸病毒和hMPV的检测。在这里，我们描述了在12年期间（1998-2010年）从8 '285名患者中收集的10'629份鼻咽抽吸物中通过DFA检测到的这些呼吸道病毒的流行病学模式。结果：增加呼吸道小核糖核酸病毒和hMPV检测将DFA阳性结果的比例从35%提高到58%（p < 0.0001）。呼吸道小核糖核酸病毒是在> 1岁的患者中检测到的最常见病毒。所研究病毒的季节模式和年龄分布与文献报道的一致。结论：DFA是一种有效、快速、灵活、廉价的检测方法。呼吸道小核糖核酸病毒和hMPV检测试剂盒的增加拓宽了其病毒检测范围。即使在“PCR时代”，DFA也是一种特别适用于儿科人群呼吸道病毒长期监测的方法。
Background: Direct immunofluorescence assays (DFA) are a rapid and inexpensive method for the detection of respiratory viruses and may therefore be used for surveillance. Few epidemiological studies have been published based solely on DFA and none included respiratory picornaviruses and human metapneumovirus (hMPV). We wished to evaluate the use of DFA for epidemiological studies with a long-term observation of respiratory viruses that includes both respiratory picornaviruses and hMPV.Methods: Since 1998 all children hospitalized with respiratory illness at the University Hospital Bern have been screened with DFA for common respiratory viruses including adenovirus, respiratory syncytial virus (RSV), influenza A and B, and parainfluenza virus 1-3. In 2006 assays for respiratory picornaviruses and hMPV were added. Here we describe the epidemiological pattern for these respiratory viruses detected by DFA in 10'629 nasopharyngeal aspirates collected from 8'285 patients during a 12-year period (1998-2010).Results: Addition of assays for respiratory picornaviruses and hMPV raised the proportion of positive DFA results from 35% to 58% (p < 0.0001). Respiratory picornaviruses were the most common viruses detected among patients > 1 year old. The seasonal patterns and age distribution for the studied viruses agreed well with those reported in the literature. In 2010, an hMPV epidemic of unexpected size was observed.Conclusions: DFA is a valid, rapid, flexible and inexpensive method. The addition of assays for respiratory picornaviruses and hMPV broadens its range of viral detection. DFA is, even in the "PCR era", a particularly adapted method for the long term surveillance of respiratory viruses in a pediatric population.