The effect of neonatal nerve injury on the expression of heat shock proteins in developing rat motoneurones
The effect of neonatal nerve injury on the expression of heat shock proteins in developing rat motoneurones
复制标题
DOI：
10.1089/089771502753754127
复制
发表时间：
2002-05-01
影响因子：
4.2
通讯作者：
Greensmith, L
中科院分区：
文献类型：
--
作者：
Kalmár, B;Burnstock, G;Greensmith, L
热休克蛋白27和热休克蛋白70的表达进行了检查，在脊髓和坐骨神经发育大鼠。利用免疫组化，我们发现，热休克蛋白27是目前在许多运动神经元出生。随着发育，染色强度增加，达到成人水平的21天，当所有坐骨运动神经元表达热休克蛋白27。在坐骨神经，热休克蛋白27强烈表达在整个出生后的发展。相反，运动神经元和坐骨神经中的hsp 70免疫反应性在出生时较弱，并且不随发育而改变。热休克蛋白的表达已被证明在应激条件下在细胞中增加，其中它们对细胞存活具有有益的影响。本研究还检测了新生儿神经损伤对hsp 27和hsp 70表达的影响。损伤后4天，运动神经元中hsp 27的染色增加，而hsp 70没有变化。然而，有一个显着的增加，热休克蛋白70染色周围的神经胶质细胞受伤的运动池，主要是在星形胶质细胞。由于新生儿神经损伤诱导运动神经元凋亡死亡，我们还研究了热休克蛋白27与凋亡标志物的共表达。通过TUNEL和caspase-3免疫反应性评估，未发现HSP 27阳性运动神经元凋亡。因此，在损伤的运动神经元中观察到的hsp 27上调可能在保护运动神经元免受神经损伤后细胞凋亡方面发挥作用。
The expression of the heat shock proteins hsp27 and hsp70 was examined in the spinal cord and sciatic nerves of developing rats. Using immunohistochemistry, we found that hsp27 is present in many motoneurones at birth. With development, the intensity of staining increases, reaching adult levels by 21 days, when all sciatic motoneurones express hsp27. In the sciatic nerve, hsp27 is strongly expressed throughout postnatal development. In contrast, hsp70 immunoreactivity in motoneurones and the sciatic nerve is weak at birth and does not change with development. The expression of heat shock proteins has been shown to increase in cells under conditions of stress, where they have beneficial effects on cell survival. The effect of neonatal nerve injury on hsp27 and hsp70 expression was also examined in this study. Four days after injury, staining for hsp27 increases in motoneurones, whereas hsp70 does not change. However, there is a significant increase in hsp70 staining in glial cells surrounding the injured motor pool, predominantly in astrocytes. Since neonatal nerve injury induces apoptotic motoneurone death, we also studied the co-expression of hsp27 with markers of apoptosis. No hsp27-positive motoneurones were found to be apoptotic, as assessed by both TUNEL and caspase-3 immunoreactivity. Therefore, it is possible that the upregulation of hsp27 observed in injured motoneurones may play a role in protecting motoneurones from apoptotic cell death following nerve injury.