Baicalein reduces hepatic fat accumulation by activating AMPK in oleic acid-induced HepG2 cells and high-fat diet-induced non-insulin-resistant mice
Baicalein reduces hepatic fat accumulation by activating AMPK in oleic acid-induced HepG2 cells and high-fat diet-induced non-insulin-resistant mice
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黄芩素通过激活油酸诱导的 HepG2 细胞和高脂饮食诱导的非胰岛素抵抗小鼠中的 AMPK 减少肝脏脂肪积累
DOI：
10.1039/c9fo02237f
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发表时间：
2020-01-01
期刊：
影响因子：
6.1
通讯作者：
Li, Jingda
中科院分区：
文献类型：
--
作者：
Sun, Wenlong;Liu, Panpan;Li, Jingda
非酒精性脂肪性肝病（NAFLD）已成为全球最常见的肝病;因此，需要一种可以限制肝脏脂肪积累的膳食补充剂。黄芩素是黄芩的主要成分，在东方和西方文化中被用作膳食补充剂，可以减少肝脏脂肪积累。然而，黄芩素发挥这种作用的详细机制尚未在体内和体外阐明。在这项研究中，我们表征了黄芩素的肝脏降脂活性，发现黄芩素通过激活AMPK和抑制SREBP 1切割来减少肝脏脂肪积累，从而抑制SREBP 1的转录活性和油酸诱导的HepG 2细胞和高脂饮食诱导的非胰岛素抵抗小鼠的肝脏脂肪合成。黄芩素通过降低TC、升高HDL C、降低LDL C、影响抗氧化活性等作用改善NAFLD。因此，黄芩素防治NAFLD的机制可能涉及多个靶点和多个途径。我们的研究支持使用黄芩素作为膳食补充剂，因为它能够减少肝脏脂肪积累和改善NAFLD相关的生化异常。
Nonalcoholic fatty liver disease (NAFLD) has become the most common liver disease worldwide; thus, a dietary supplement that can restrict hepatic fat accumulation is needed. Baicalein, a major component of Scutellaria baicalensis, is used as a dietary supplement in Eastern and Western cultures and can reduce hepatic fat accumulation. However, the detailed mechanism by which baicalein exerts this effect has yet to be elucidated in vivo and in vitro. In this study, we characterized the hepatic fat-lowering activity of baicalein and found that baicalein reduced hepatic fat accumulation by activating AMPK and suppressing SREBP1 cleavage, thus consequently inhibiting the transcriptional activity of SREBP1 and the synthesis of hepatic fat in oleic acid-induced HepG2 cells and high-fat diet-induced non-insulin-resistant mice. Moreover, baicalein improved NAFLD by decreasing TC, increasing HDLC, decreasing LDLC, affecting antioxidant activity, and exerting other effects. Therefore, the mechanism of baicalein with regard to NAFLD prevention and treatment might involve effects on multiple targets and pathways. Our study supports the use of baicalein as a dietary supplement due to its ability to reduce hepatic fat accumulation and to ameliorate NAFLD-related biochemical abnormalities.