Surface hypothermia predicts murine mortality in the intragastric Vibrio vulnificus infection model
Surface hypothermia predicts murine mortality in the intragastric Vibrio vulnificus infection model
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DOI：
10.1186/s12866-017-1045-z
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发表时间：
2017-06-19
期刊：
影响因子：
4.2
通讯作者：
Satchell, Karla J. F.
中科院分区：
文献类型：
--
作者：
Gavin, Hannah E.;Satchell, Karla J. F.
背景：革兰氏阴性细菌创伤弧菌可导致严重的疾病，在人类谁消耗未煮熟的，受污染的海鲜。为了在实验室中研究食源性创伤弧菌病，小鼠毒力研究主要使用死亡作为主要实验终点，因为基于行为的濒死状态并不能始终预测致死率。本研究评估了创伤弧菌毒力研究期间腹表面温度（VST）及其与小鼠存活率的相关性，作为一种有效、人道的替代方法。方法：每2小时测量一次胃内接种创伤弧菌的小鼠的VST，持续24小时，并分析最小VST的数据以预测致死结果。结果：与模拟感染对照动物的相对稳定的VST相反，感染创伤弧菌的小鼠表现出低温，最低温度发生在接种后8至12小时。继续死亡的小鼠的最小VST显著低于存活小鼠。VST
Background: The Gram-negative bacterium Vibrio vulnificus can cause severe disease in humans who consume undercooked, contaminated seafood. To study food-borne V. vulnificus disease in the laboratory, mouse virulence studies predominantly use death as the primary experimental endpoint because behaviorally based moribund status does not consistently predict lethality. This study assessed ventral surface temperature (VST) and its association with mouse survival during V. vulnificus virulence studies as an efficacious, humane alternative.Methods: VST of mice intragastrically inoculated with V. vulnificus was measured every 2-h for 24 h and data for minimal VST analyzed for prediction of lethal outcome.Results: In contrast to the relatively stable VST of mock-infected control animals, mice infected with V. vulnificus exhibited hypothermia with minima occurring 8 to 12 h post-inoculation. The minimum VST of mice that proceeded to death was significantly lower than that of surviving mice. VST